GenEPi: Piezo1-based fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution

Yaganoglu, Sine, Helassa, Nordine ORCID: 0000-0003-3743-1886, Gaub, Benjamin, Welling, Maaike, Shi, Jian, Müller, Daniel, Török, Katalin and Pantazis, Periklis ORCID: 0000-0002-8367-9332 (2019) GenEPi: Piezo1-based fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution. Biorxiv. 702423-.

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Official URL: http://dx.doi.org/10.1101/702423

Abstract

Mechanosensing is a ubiquitous process to translate external mechanical stimuli into biological responses during development, homeostasis, and disease. However, non-invasive investigation of cellular mechanosensing in complex and intact live tissue remains challenging. Here, we developed GenEPi, a genetically-encoded fluorescent intensiometric reporter for mechanical stimuli based on Piezo1, an essential mechanosensitive ion channel found in vertebrates. We show that GenEPi has high specificity and spatiotemporal resolution for Piezo1-dependent mechanical stimuli, exemplified by resolving repetitive mechanical stimuli of spontaneously contracting cardiomyocytes within microtissues, in a non-invasive manner.

Item Type:	Article
Uncontrolled Keywords:	Bioengineering, 1.1 Normal biological development and functioning, 1 Underpinning research
Depositing User:	Symplectic Admin
Date Deposited:	02 Sep 2020 15:16
Last Modified:	15 Mar 2024 06:38
DOI:	10.1101/702423
Open Access URL:	https://www.biorxiv.org/content/10.1101/702423v1.f...
Related URLs:	Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3099700