Yaganoglu, Sine, Helassa, Nordine ORCID: 0000-0003-3743-1886, Gaub, Benjamin, Welling, Maaike, Shi, Jian, Müller, Daniel, Török, Katalin and Pantazis, Periklis ORCID: 0000-0002-8367-9332
(2019)
GenEPi: Piezo1-based fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution.
Biorxiv.
702423-.
Text
GenEPi- Piezo1-based fluorescent reporter for visualizing 2 mechanical stimuli with high spatiotemporal resolution.pdf - Submitted version Download (3MB) | Preview |
Abstract
Mechanosensing is a ubiquitous process to translate external mechanical stimuli into biological responses during development, homeostasis, and disease. However, non-invasive investigation of cellular mechanosensing in complex and intact live tissue remains challenging. Here, we developed GenEPi, a genetically-encoded fluorescent intensiometric reporter for mechanical stimuli based on Piezo1, an essential mechanosensitive ion channel found in vertebrates. We show that GenEPi has high specificity and spatiotemporal resolution for Piezo1-dependent mechanical stimuli, exemplified by resolving repetitive mechanical stimuli of spontaneously contracting cardiomyocytes within microtissues, in a non-invasive manner.
Item Type: | Article |
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Uncontrolled Keywords: | Bioengineering, 1.1 Normal biological development and functioning, 1 Underpinning research |
Depositing User: | Symplectic Admin |
Date Deposited: | 02 Sep 2020 15:16 |
Last Modified: | 15 Mar 2024 06:38 |
DOI: | 10.1101/702423 |
Open Access URL: | https://www.biorxiv.org/content/10.1101/702423v1.f... |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3099700 |