Amrun, Siti Naqiah, Lee, Cheryl Yi-Pin, Lee, Bernett, Fong, Siew-Wai, Young, Barnaby Edward, Chee, Rhonda Sin-Ling, Yeo, Nicholas Kim-Wah, Torres-Ruesta, Anthony, Carissimo, Guillaume, Poh, Chek Meng et al (show 14 more authors)
(2020)
Linear B-cell epitopes in the spike and nucleocapsid proteins as markers of SARS-CoV-2 exposure and disease severity.
EBIOMEDICINE, 58.
102911-.
|
Text
PIIS2352396420302863.pdf - Published version Download (1MB) | Preview |
Abstract
<h4>Background</h4>Given the unceasing worldwide surge in COVID-19 cases, there is an imperative need to develop highly specific and sensitive serology assays to define exposure to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2).<h4>Methods</h4>Pooled plasma samples from PCR positive COVID-19 patients were used to identify linear B-cell epitopes from a SARS-CoV-2 peptide library of spike (S), envelope (E), membrane (M), and nucleocapsid (N) structural proteins by peptide-based ELISA. Hit epitopes were further validated with 79 COVID-19 patients with different disease severity status, 13 seasonal human CoV, 20 recovered SARS patients and 22 healthy donors.<h4>Findings</h4>Four immunodominant epitopes, S14P5, S20P2, S21P2 and N4P5, were identified on the S and N viral proteins. IgG responses to all identified epitopes displayed a strong detection profile, with N4P5 achieving the highest level of specificity (100%) and sensitivity (>96%) against SARS-CoV-2. Furthermore, the magnitude of IgG responses to S14P5, S21P2 and N4P5 were strongly associated with disease severity.<h4>Interpretation</h4>IgG responses to the peptide epitopes can serve as useful indicators for the degree of immunopathology in COVID-19 patients, and function as higly specific and sensitive sero-immunosurveillance tools for recent or past SARS-CoV-2 infections. The flexibility of these epitopes to be used alone or in combination will allow for the development of improved point-of-care-tests (POCTs).<h4>Funding</h4>Biomedical Research Council (BMRC), the A*ccelerate GAP-funded project (ACCL/19-GAP064-R20H-H) from Agency of Science, Technology and Research (A*STAR), and National Medical Research Council (NMRC) COVID-19 Research fund (COVID19RF-001) and CCGSFPOR20002. ATR is supported by the Singapore International Graduate Award (SINGA), A*STAR.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Epitopes, SARS-CoV-2, COVID-19, Patients, Biomarkers |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 01 Oct 2020 09:40 |
| Last Modified: | 18 Jan 2023 23:30 |
| DOI: | 10.1016/j.ebiom.2020.102911 |
| Open Access URL: | http://doi.org/10.1016/j.ebiom.2020.102911 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3103211 |
Dimensions
Dimensions
