Fleming, Jason C 
ORCID: 0000-0001-7963-1224, Woo, Jeongmin, Moutasim, Karwan, Mellone, Massimiliano, Frampton, Steven J, Mead, Abbie, Ahmed, Waseem, Wood, Oliver, Robinson, Hollie, Ward, Matthew et al (show 6 more authors)
(2019)
HPV, tumour metabolism and novel target identification in head and neck squamous cell carcinoma.
British Journal of Cancer, 120 (3).
pp. 356-367.
|
Text
HPV, tumour metabolism and novel target identification in head and neck squamous cell carcinoma.pdf - Published Version Download (1MB) | Preview |
Abstract
Background Metabolic changes in tumour cells are used in clinical imaging and may provide potential therapeutic targets. Human papillomavirus (HPV) status is important in classifying head and neck cancers (HNSCC), identifying a distinct clinical phenotype; metabolic differences between these HNSCC subtypes remain poorly understood. Methods We used RNA sequencing to classify the metabolic expression profiles of HPV+ve and HPV−ve HNSCC, performed a meta-analysis on FDG-PET imaging characteristics and correlated results with in vitro extracellular flux analysis of HPV−ve and HPV+ve HNSCC cell lines. The monocarboxylic acid transporter-1 (MCT1) was identified as a potential metabolic target and tested in functional assays. Results Specific metabolic profiles were associated with HPV status, not limited to carbohydrate metabolism. There was dominance of all energy pathways in HPV-negative disease, with elevated expression of genes associated with glycolysis and oxidative phosphorylation. In vitro analysis confirmed comparative increased rates of oxidative phosphorylation and glycolysis in HPV-negative cell lines. PET SUV(max) scores however were unable to reliably differentiate between HPV-positive and HPV-negative tumours. MCT1 expression was significantly increased in HPV-negative tumours, and inhibition suppressed tumour cell invasion, colony formation and promoted radiosensitivity. Conclusion HPV-positive and negative HNSCC have different metabolic profiles which may have potential therapeutic applications.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Cell Line, Tumor, Humans, Papillomaviridae, Papillomavirus Infections, Neoplasm Invasiveness, Monocarboxylic Acid Transporters, Symporters, Positron-Emission Tomography, Sequence Analysis, RNA, Gene Expression Regulation, Neoplastic, Oxidative Phosphorylation, Glycolysis, Radiation Tolerance, Squamous Cell Carcinoma of Head and Neck |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 08 Oct 2020 10:23 |
| Last Modified: | 18 Jan 2023 23:29 |
| DOI: | 10.1038/s41416-018-0364-7 |
| Open Access URL: | https://www.nature.com/articles/s41416-018-0364-7 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3103873 |
Dimensions
Dimensions
