Immunopathogenesis of infectious bronchitis virus Q1 in specific pathogen free chicks

Manswr, B, Ball, C ORCID: 0000-0001-8525-8382, Forrester, A, Chantrey, J ORCID: 0000-0002-4801-7034 and Ganapathy, K ORCID: 0000-0002-9401-3560 (2020) Immunopathogenesis of infectious bronchitis virus Q1 in specific pathogen free chicks. Microbial Pathogenesis, 149. p. 104535.

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Official URL: http://dx.doi.org/10.1016/j.micpath.2020.104535

Abstract

The immunopathogenesis of avian coronavirus, infectious bronchitis virus (IBV) Q1, was investigated in specific pathogen free chicks. Following infection, chicks exhibited respiratory clinical signs and reduced body weight. Oropharyngeal (OP) and cloacal (CL) swabs were collected at intervals and found to be RT-PCR positive, with a greater number of partial-S1 amino acid changes noted in CL swabs compared to OP swabs. In tissue samples, IBV viral load peaked 9 days post infection (dpi) in the trachea and kidneys, and 14 dpi in the proventriculus. At 28 dpi, ELISA data showed that 63% of infected chicks seroconverted. There was significantly higher mRNA up-regulation of IFN-α, TLR3, MDA5, LITAF, IL-1β and IL-6 in the trachea compared to the kidneys. Findings presented here demonstrate that this Q1 isolate induces greater lesions and host innate immune responses in chickens' tracheas compared to the kidneys.

Item Type:	Article
Additional Information:	## TULIP Type: Conference Proceedings (contribution) ##
Uncontrolled Keywords:	Infectious bronchitis virus, Avian coronavirus, Specific pathogen free, Pathogenesis, Immune responses, Gene transcription
Depositing User:	Symplectic Admin
Date Deposited:	19 Oct 2020 08:46
Last Modified:	18 Jan 2023 23:29
DOI:	10.1016/j.micpath.2020.104535
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3103891