Development of Solid-Phase RPA on a Lateral Flow Device for the Detection of Pathogens Related to Sepsis

Heeroma, Alice Jane and Gwenin, Christopher ORCID: 0000-0003-0363-8168 (2020) Development of Solid-Phase RPA on a Lateral Flow Device for the Detection of Pathogens Related to Sepsis. SENSORS, 20 (15). E4182-.

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Official URL: http://dx.doi.org/10.3390/s20154182

Abstract

Population extended life expectancy has significantly increased the risk of septic shock in an ageing population. Sepsis affects roughly 20 million people every year, resulting in over 11 million deaths. The need for faster more accurate diagnostics and better management is therefore paramount in the fight to prevent these avoidable deaths. Here we report the development of a POC device with the ability to identify a broad range of pathogens on a lateral flow platform. Namely Gram-positive and Gram-negative bacteria. The simple to use laboratory device has the potential to be automated, thus enabling an operator to carry out solid-phase lysis and room temperature RPA in situ, providing accurate results in hours rather than days. Results show there is a potential for a fully automated device in which concepts described in this paper can be integrated into a lateral flow device.

Item Type:	Article
Uncontrolled Keywords:	sepsis, lateral flow immunoassay, solid phase RPA, point-of-care, bacterial infections
Divisions:	Faculty of Science and Engineering > School of Physical Sciences
Depositing User:	Symplectic Admin
Date Deposited:	30 Oct 2020 08:47
Last Modified:	16 Jun 2023 12:01
DOI:	10.3390/s20154182
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3105513