Identification of tissue-dependent proteins in knee OA synovial fluid

Timur, UT, Jahr, H, Anderson, J ORCID: 0000-0003-0489-7997, Green, DC, Emans, PJ, Smagul, A, van Rhijn, LW, Peffers, MJ ORCID: 0000-0001-6979-0440 and Welting, TJM (2021) Identification of tissue-dependent proteins in knee OA synovial fluid. OSTEOARTHRITIS AND CARTILAGE, 29 (1). pp. 124-133.

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Abstract

<h4>Objective</h4>For many proteins from osteoarthritic synovial fluid, their intra-articular tissue of origin remains unknown. In this study we performed comparative proteomics to identify osteoarthritis-specific and joint tissue-dependent secreted proteins that may serve as candidates for osteoarthritis biomarker development on a tissue-specific basis.<h4>Design</h4>Protein secretomes of cartilage, synovium, Hoffa's fat pad and meniscus from knee osteoarthritis patients were determined using liquid chromatography tandem mass spectrometry, followed by label-free quantification. Validation of tissue-dependent protein species was conducted by ELISA on independent samples. Differential proteomes of osteoarthritic and non-osteoarthritic knee synovial fluids were obtained via similar proteomics approach, followed by ELISA validation.<h4>Results</h4>Proteomics revealed 64 proteins highly secreted from cartilage, 94 from synovium, 37 from Hoffa's fat pad and 21 from meniscus. Proteomic analyses of osteoarthritic vs non-osteoarthritic knee synovial fluid revealed 70 proteins with a relatively higher abundance and 264 proteins with a relatively lower abundance in osteoarthritic synovial fluid. Of the 70 higher abundance proteins, 23 were amongst the most highly expressed in the secretomes of a specific intra-articular tissue measured. Tissue-dependent release was validated for SLPI, C8, CLU, FN1, RARRES2, MATN3, MMP3 and TNC. Abundance in synovial fluid of tissue-dependent proteins was validated for IGF2, AHSG, FN1, CFB, KNG and C8.<h4>Conclusions</h4>We identified proteins with a tissue-dependent release from intra-articular human knee OA tissues. A number of these proteins also had an osteoarthritis-specific abundance in knee synovial fluid. These proteins may serve as novel candidates for osteoarthritis biomarker development on a tissue-specific basis.

Item Type:	Article
Uncontrolled Keywords:	Synovial fluid, Knee joint, Tissue-dependent, Biomarker, Osteoarthritis, Proteomics
Depositing User:	Symplectic Admin
Date Deposited:	16 Nov 2020 10:15
Last Modified:	18 Jan 2023 23:21
DOI:	10.1016/j.joca.2020.09.005
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3106825