16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture in the Investigation of Suspected Bacterial Keratitis

Somerville, Tobi F, Corless, Caroline E, Sueke, Henri, Neal, Timothy and Kaye, Stephen B ORCID: 0000-0003-0390-0592 (2020) 16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture in the Investigation of Suspected Bacterial Keratitis. TRANSLATIONAL VISION SCIENCE & TECHNOLOGY, 9 (13). 2-.

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Official URL: http://dx.doi.org/10.1167/tvst.9.13.2

Abstract

<h4>Purpose</h4>The purpose of this study was to compare conventional diagnostic culture (CDC) to 16S ribosomal RNA polymerase chain reaction (PCR) analysis for diagnosing bacterial keratitis.<h4>Methods</h4>Samples collected from 100 consecutive patients presenting to the Royal Liverpool University Hospital with bacterial keratitis were processed using CDC and 16S PCR analysis.<h4>Results</h4>The overall detection rate using both methods was 36%. Of these, 72.2% (26/36) were detected by PCR and 63.9% (23/36) isolated by CDC (<i>P</i> = 0.62). Using a combination of both PCR and CDC increased the detection rate for pathogenic bacteria by 13% compared to using CDC alone (<i>P</i> = 0.04). In CDC negative samples, 16S PCR identified more pathogens than CDC in 16S PCR negative samples. Neither order of sample collection nor prior antimicrobial use affected the detection rate.<h4>Conclusions</h4>16S rRNA gene PCR performed in addition to CDC on corneal samples from patients with clinically suspected bacterial keratitis led to additional pathogen detection.<h4>Translational relevance</h4>16S rRNA gene PCR should be developed to become an additional part of clinical service for patients with bacterial keratitis rather than used in isolation.

Item Type:	Article
Uncontrolled Keywords:	bacterial keratitis, 16S polymerase chain reaction (PCR), conventional diagnostic culture
Depositing User:	Symplectic Admin
Date Deposited:	15 Dec 2020 09:15
Last Modified:	18 Jan 2023 23:06
DOI:	10.1167/tvst.9.13.2
Open Access URL:	https://doi.org/10.1167/tvst.9.13.2
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3110437