Amplicon based MinION sequencing of SARS-CoV-2 and metagenomic characterisation of nasopharyngeal swabs from patients with COVID-19

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Moore, Shona ORCID: 0000-0001-8610-2806, Penrice-Randal, Rebekah ORCID: 0000-0002-0653-2097, Alruwaili, Muhannad, Dong, Xiaofeng, Pullan, Steven, Carter, Daniel, Bewley, Kevin ORCID: 0000-0002-9078-6734, Zhao, Qin, Sun, Yani, Hartley, Catherine et al (show 20 more authors) , Zhou, En-min, Solomon, Tom, Beadsworth, Michael, Cruise, James, Bogaert, Debby, Crook, Derrick ORCID: 0000-0002-0590-2850, Matthews, David ORCID: 0000-0003-4611-8795, Davidson, Andrew, Mahmood, Zana ORCID: 0000-0001-6000-6648, Aljabr, Waleed, Druce, Julian, Vipond, Richard, Ng, Lisa, Renia, Laurent, Openshaw, Peter, Baillie, Kenneth, Carroll, Miles, Semple, Malcolm ORCID: 0000-0001-9700-0418, Turtle, Lance ORCID: 0000-0002-0778-1693 and Hiscox, Julian (2020) Amplicon based MinION sequencing of SARS-CoV-2 and metagenomic characterisation of nasopharyngeal swabs from patients with COVID-19. medrxiv. 2020.03.05.20032011-.

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Abstract

COVID-19 is a complex disease phenotype where the underlying microbiome could influence morbidity and mortality. Amplicon and metagenomic MinION based sequencing was used to rapidly (within 8 hours) identify SARS-CoV-2 and assess the microbiome in nasopharyngeal swabs obtained from patients with COVID-19 by the ISARIC 4C consortium.

Item Type:	Article
Uncontrolled Keywords:	Pneumonia, Genetics, Human Genome, Lung, 3 Good Health and Well Being
Depositing User:	Symplectic Admin
Date Deposited:	08 Jan 2021 15:16
Last Modified:	14 Mar 2024 18:41
DOI:	10.1101/2020.03.05.20032011
Open Access URL:	https://doi.org/10.1101/2020.03.05.20032011
Related URLs:	Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3112074