Postnatal mechanical loading drives adaptation of tissues primarily through modulation of the non-collagenous matrix

Zamboulis, DE ORCID: 0000-0003-2839-7620, Thorpe, CT, Ashraf Kharaz, Y ORCID: 0000-0003-4925-0206, Birch, HL, Screen, HRC and Clegg, PD ORCID: 0000-0003-0632-0032 (2020) Postnatal mechanical loading drives adaptation of tissues primarily through modulation of the non-collagenous matrix. eLife, 2020 (9). e58075-.

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Abstract

Mature connective tissues demonstrate highly specialised properties, remarkably adapted to meet their functional requirements. Tissue adaptation to environmental cues can occur throughout life and poor adaptation commonly results in injury. However, the temporal nature and drivers of functional adaptation remain undefined. Here, we explore functional adaptation and specialisation of mechanically loaded tissues using tendon; a simple aligned biological composite, in which the collagen (fibre phase) and surrounding predominantly non-collagenous matrix (matrix phase) can be interrogated independently. Using an equine model of late development, we report the first phase-specific analysis of biomechanical, structural and compositional changes seen in functional adaptation, demonstrating adaptation occurs postnatally, following mechanical loading, and is almost exclusively localised to the non-collagenous matrix phase. These novel data redefine adaptation in connective tissue, highlighting the fundamental importance of non-collagenous matrix and suggesting that regenerative medicine strategies should change focus from the fibrous to the non-collagenous matrix phase of tissue.

Item Type:	Article
Uncontrolled Keywords:	Tendons, Connective Tissue, Extracellular Matrix, Animals, Horses, Tendon Injuries, Collagen, Transforming Growth Factor beta, Proteome, Regenerative Medicine, Adaptation, Physiological, Stress, Mechanical, Biomechanical Phenomena
Depositing User:	Symplectic Admin
Date Deposited:	14 Jan 2021 08:54
Last Modified:	18 Jan 2023 23:03
DOI:	10.7554/elife.58075
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3113525

Available Versions of this Item

• Postnatal mechanical loading drives adaptation of tissues primarily through modulation of the non-collagenous matrix. (deposited 23 Oct 2020 09:15)
  • Postnatal mechanical loading drives adaptation of tissues primarily through modulation of the non-collagenous matrix. (deposited 14 Jan 2021 08:54) [Currently Displayed]