In silico prediction of structure and function for a large family of transmembrane proteins that includes human Tmem41b.



Mesdaghi, Shahram, Murphy, David L, Sánchez Rodríguez, Filomeno ORCID: 0000-0002-6377-7269, Burgos-Mármol, J Javier ORCID: 0000-0003-1861-2657 and Rigden, Daniel J ORCID: 0000-0002-7565-8937
(2020) In silico prediction of structure and function for a large family of transmembrane proteins that includes human Tmem41b. F1000Research, 9. 1395-.

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Abstract

<b>Background:</b> Recent strides in computational structural biology have opened up an opportunity to understand previously uncharacterised proteins.  The under-representation of transmembrane proteins in the Protein Data Bank highlights the need to apply new and advanced bioinformatics methods to shed light on their structure and function.  This study focuses on a family of transmembrane proteins containing the Pfam domain PF09335 ('SNARE_ASSOC'/ 'VTT '/'Tvp38'/'DedA'). One prominent member, Tmem41b, has been shown to be involved in early stages of autophagosome formation and is vital in mouse embryonic development as well as being identified as a viral host factor of SARS-CoV-2. <b>Methods:</b> We used evolutionary covariance-derived information to construct and validate <i>ab initio</i> models, make domain boundary predictions and infer local structural features.  <b>Results:</b> The results from the structural bioinformatics analysis of Tmem41b and its homologues showed that they contain a tandem repeat that is clearly visible in evolutionary covariance data but much less so by sequence analysis.  Furthermore, cross-referencing of other prediction data with covariance analysis showed that the internal repeat features two-fold rotational symmetry.  <i>Ab initio</i> modelling of Tmem41b and homologues reinforces these structural predictions.  Local structural features predicted to be present in Tmem41b were also present in Cl <sup>-</sup>/H <sup>+</sup> antiporters.  <b>Conclusions:</b> The results of this study strongly point to Tmem41b and its homologues being transporters for an as-yet uncharacterised substrate and possibly using H <sup>+</sup> antiporter activity as its mechanism for transport.

Item Type: Article
Uncontrolled Keywords: Animals, Humans, Mice, Membrane Proteins, Embryonic Development, Computer Simulation, COVID-19, SARS-CoV-2
Depositing User: Symplectic Admin
Date Deposited: 21 Jan 2021 10:58
Last Modified: 18 Jan 2023 23:02
DOI: 10.12688/f1000research.27676.2
Open Access URL: http://doi.org/10.12688/f1000research.27676.1
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3114401