Zhu, Yanfang Peipei, Padgett, Lindsey, Dinh, Huy Q, Marcovecchio, Paola, Blatchley, Amy, Wu, Runpei, Ehinger, Erik, Kim, Cheryl, Mikulski, Zbigniew, Seumois, Gregory et al (show 3 more authors)
(2018)
Identification of an Early Unipotent Neutrophil Progenitor with Pro-tumoral Activity in Mouse and Human Bone Marrow.
Cell Reports, 24 (9).
pp. 2329-2341.
Text
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Abstract
Neutrophils are short-lived cells that play important roles in both health and disease. Neutrophils and monocytes originate from the granulocyte monocyte progenitor (GMP) in bone marrow; however, unipotent neutrophil progenitors are not well defined. Here, we use cytometry by time of flight (CyTOF) and single-cell RNA sequencing (scRNA-seq) methodologies to identify a committed unipotent early-stage neutrophil progenitor (NeP) in adult mouse bone marrow. Importantly, we found a similar unipotent NeP (hNeP) in human bone marrow. Both NeP and hNeP generate only neutrophils. NeP and hNeP both significantly increase tumor growth when transferred into murine cancer models, including a humanized mouse model. hNeP are present in the blood of treatment-naive melanoma patients but not of healthy subjects. hNeP can be readily identified by flow cytometry and could be used as a biomarker for early cancer discovery. Understanding the biology of hNeP should allow the development of new therapeutic targets for neutrophil-related diseases, including cancer.
Item Type: | Article |
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Uncontrolled Keywords: | neutrophil progenitor, CyTOF, scRNA-seq, granulopoiesis, melanoma, sarcoma, T cell suppression |
Depositing User: | Symplectic Admin |
Date Deposited: | 12 Feb 2021 08:26 |
Last Modified: | 18 Jan 2023 23:00 |
DOI: | 10.1016/j.celrep.2018.07.097 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3115512 |