Identification of an Early Unipotent Neutrophil Progenitor with Pro-tumoral Activity in Mouse and Human Bone Marrow



Zhu, Yanfang Peipei, Padgett, Lindsey, Dinh, Huy Q, Marcovecchio, Paola, Blatchley, Amy, Wu, Runpei, Ehinger, Erik, Kim, Cheryl, Mikulski, Zbigniew, Seumois, Gregory
et al (show 3 more authors) (2018) Identification of an Early Unipotent Neutrophil Progenitor with Pro-tumoral Activity in Mouse and Human Bone Marrow. Cell Reports, 24 (9). pp. 2329-2341.

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Abstract

Neutrophils are short-lived cells that play important roles in both health and disease. Neutrophils and monocytes originate from the granulocyte monocyte progenitor (GMP) in bone marrow; however, unipotent neutrophil progenitors are not well defined. Here, we use cytometry by time of flight (CyTOF) and single-cell RNA sequencing (scRNA-seq) methodologies to identify a committed unipotent early-stage neutrophil progenitor (NeP) in adult mouse bone marrow. Importantly, we found a similar unipotent NeP (hNeP) in human bone marrow. Both NeP and hNeP generate only neutrophils. NeP and hNeP both significantly increase tumor growth when transferred into murine cancer models, including a humanized mouse model. hNeP are present in the blood of treatment-naive melanoma patients but not of healthy subjects. hNeP can be readily identified by flow cytometry and could be used as a biomarker for early cancer discovery. Understanding the biology of hNeP should allow the development of new therapeutic targets for neutrophil-related diseases, including cancer.

Item Type: Article
Uncontrolled Keywords: neutrophil progenitor, CyTOF, scRNA-seq, granulopoiesis, melanoma, sarcoma, T cell suppression
Depositing User: Symplectic Admin
Date Deposited: 12 Feb 2021 08:26
Last Modified: 18 Jan 2023 23:00
DOI: 10.1016/j.celrep.2018.07.097
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3115512