Isolation and characterisation of bacteriophages with activity against invasive non-typhoidal Salmonella causing bloodstream infection in Malawi

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Rodwell, Ella, Wenner, Nicolas, Pulford, Caisey, Cai, Yueyi, Bowers-Barnard, Arthur, Beckett, Alison ORCID: 0000-0001-8377-325X, Rigby, Jonathan, Picton, David, Blower, Tim, Feasey, Nicholas et al (show 2 more authors) , Hinton, Jay and Perez Sepulveda, Blanca ORCID: 0000-0002-2891-4010 (2021) Isolation and characterisation of bacteriophages with activity against invasive non-typhoidal Salmonella causing bloodstream infection in Malawi. Viruses, 13 (3). 478-.

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Abstract

In recent years, novel lineages of invasive non-typhoidal <i>Salmonella</i> (iNTS) serovars Typhimurium and Enteritidis have been identified in patients with bloodstream infection in Sub-Saharan Africa. Here, we isolated and characterised 32 phages capable of infecting <i>S</i>. Typhimurium and <i>S</i>. Enteritidis, from water sources in Malawi and the UK. The phages were classified in three major phylogenetic clusters that were geographically distributed. In terms of host range, Cluster 1 phages were able to infect all bacterial hosts tested, whereas Clusters 2 and 3 had a more restricted profile. Cluster 3 contained two sub-clusters, and 3.b contained the most novel isolates. This study represents the first exploration of the potential for phages to target the lineages of <i>Salmonella</i> that are responsible for bloodstream infections in Sub-Saharan Africa.

Item Type:	Article
Uncontrolled Keywords:	Enteritidis, Typhimurium, Malawi, environmental phage
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	16 Mar 2021 09:10
Last Modified:	18 Nov 2023 03:03
DOI:	10.3390/v13030478
Open Access URL:	https://www.mdpi.com/1999-4915/13/3/478
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3117373

Available Versions of this Item

• Isolation and characterisation of bacteriophages with activity against invasive non-typhoidal Salmonella causing bloodstream infection in Malawi. (deposited 22 Feb 2021 16:23)
  • Isolation and characterisation of bacteriophages with activity against invasive non-typhoidal Salmonella causing bloodstream infection in Malawi. (deposited 16 Mar 2021 09:10) [Currently Displayed]