A haemagglutination test for rapid detection of antibodies to SARS-CoV-2

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Townsend, Alain, Rijal, Pramila, Xiao, Julie, Tan, Tiong Kit, Huang, Kuan-Ying A, Schimanski, Lisa, Huo, Jiandong, Gupta, Nimesh, Rahikainen, Rolle, Matthews, Philippa C et al (show 53 more authors) , Crook, Derrick, Hoosdally, Sarah, Dunachie, Susanna, Barnes, Eleanor, Street, Teresa, Conlon, Christopher P, Frater, John, Arancibia-Carcamo, Carolina V, Rudkin, Justine, Stoesser, Nicole, Karpe, Fredrik, Neville, Matthew, Ploeg, Rutger, Oliveira, Marta, Roberts, David J, Lamikanra, Abigail A, Tsang, Hoi Pat, Bown, Abbie, Vipond, Richard, Mentzer, Alexander J, Knight, Julian C, Kwok, Andrew J, Screaton, Gavin R, Mongkolsapaya, Juthathip, Dejnirattisai, Wanwisa, Supasa, Piyada, Klenerman, Paul, Dold, Christina, Baillie, J Kenneth, Moore, Shona C ORCID: 0000-0001-8610-2806, Openshaw, Peter JM, Semple, Malcolm G ORCID: 0000-0001-9700-0418, Turtle, Lance CW ORCID: 0000-0002-0778-1693, Ainsworth, Mark, Allcock, Alice, Beer, Sally, Bibi, Sagida, Skelly, Donal, Stafford, Lizzy, Jeffrey, Katie, O'Donnell, Denise, Clutterbuck, Elizabeth, Espinosa, Alexis, Mendoza, Maria, Georgiou, Dominique, Lockett, Teresa, Martinez, Jose, Perez, Elena, Sanchez, Veronica Gallardo, Scozzafava, Giuseppe, Sobrinodiaz, Alberto, Thraves, Hannah and Joly, Etienne (2021) A haemagglutination test for rapid detection of antibodies to SARS-CoV-2. NATURE COMMUNICATIONS, 12 (1). 1951-.

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Abstract

Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world.

Item Type:	Article
Uncontrolled Keywords:	Humans, Antibodies, Monoclonal, Antibodies, Viral, Enzyme-Linked Immunosorbent Assay, Agglutination Tests, Hemagglutination Tests, Sensitivity and Specificity, Polymerase Chain Reaction, Point-of-Care Systems, Spike Glycoprotein, Coronavirus, Seroconversion, COVID-19, SARS-CoV-2, COVID-19 Testing
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	09 Apr 2021 08:16
Last Modified:	18 Jan 2023 22:53
DOI:	10.1038/s41467-021-22045-y
Open Access URL:	https://www.nature.com/articles/s41467-021-22045-y
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3118758