<i>Spiroplasma</i> Isolated From Third-Generation Laboratory Colony <i>Ixodes persulcatus</i> Ticks


Beliavskaia, Alexandra, Honig, Vaclav, Erhart, Jan, Vyhlidalova, Tereza, Palus, Martin, Cerny, Jiri, Kozlova, Irina, Ruzek, Daniel, Palomar, Ana M and Bell-Sakyi, Lesley ORCID: 0000-0002-7305-0477 (2021) <i>Spiroplasma</i> Isolated From Third-Generation Laboratory Colony <i>Ixodes persulcatus</i> Ticks. FRONTIERS IN VETERINARY SCIENCE, 8. 659786-.

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Abstract

<i>Spiroplasma</i> are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected <i>Ixodes persulcatus</i> have been reported to harbour <i>Spiroplasma</i>, but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated <i>Spiroplasma</i> from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an <i>I. persulcatus</i> laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of <i>Spiroplasma</i> by genus-specific PCR amplification of fragments of the 16S rRNA and <i>rpoB</i> genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published <i>Spiroplasma</i> isolated from field-collected <i>I. persulcatus</i>. Our study shows that <i>Spiroplasma</i> endosymbionts persist at high prevalence in colonised <i>I. persulcatus</i> through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.

Item Type: Article
Uncontrolled Keywords: tick cell line, endosymbiont, Spiroplasma, tick colony, Ixodes persulcatus
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User: Symplectic Admin
Date Deposited: 21 Apr 2021 14:58
Last Modified: 19 Oct 2023 08:42
DOI: 10.3389/fvets.2021.659786
Open Access URL: https://doi.org/10.3389/fvets.2021.659786
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3120066
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