Sensitive detection of total anti-Spike antibodies and isotype switching in asymptomatic and symptomatic individuals with COVID-19

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Goh, Yun Shan, Chavatte, Jean-Marc, Jieling, Alicia Lim, Lee, Bernett, Hor, Pei Xiang, Amrun, Siti Naqiah, Lee, Cheryl Yi-Pin, Chee, Rhonda Sin-Ling, Wang, Bei, Lee, Chia Yin et al (show 14 more authors) , Ngoh, Eve Zhi Xian, Wang, Cheng-I, Young, Barnaby Edward, Tambyah, Paul A, Kalimuddin, Shirin, Pada, Surinder, Tan, Seow-Yen, Sun, Louisa Jin, Chen, Mark I-Cheng, Leo, Yee-Sin, Lye, David C, Ng, Lisa FP ORCID: 0000-0003-4071-5222, Lin, Raymond Tzer Pin and Renia, Laurent (2021) Sensitive detection of total anti-Spike antibodies and isotype switching in asymptomatic and symptomatic individuals with COVID-19. CELL REPORTS MEDICINE, 2 (2). 100193-.

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Abstract

Early detection of infection is crucial to limit the spread of coronavirus disease 2019 (COVID-19). Here we develop a flow cytometry-based assay to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein antibodies in individuals with COVID-19. The assay detects specific immunoglobulin M (IgM), IgA, and IgG in individuals with COVID-19 and also acquisition of all IgG subclasses, with IgG1 being the most dominant. The antibody response is significantly higher at a later stage of infection. Furthermore, asymptomatic individuals with COVID-19 also develop specific IgM, IgA, and IgG, with IgG1 being the most dominant subclass. Although the antibody levels are lower in asymptomatic infection, the assay is highly sensitive and detects 97% of asymptomatic infections. These findings demonstrate that the assay can be used for serological analysis of symptomatic and asymptomatic infections, which may otherwise remain undetected.

Item Type:	Article
Additional Information:	Source info: CR-MEDICINE-D-20-00412
Uncontrolled Keywords:	Humans, Immunoglobulin G, Antibodies, Viral, Immunologic Tests, Flow Cytometry, Immunoglobulin Class Switching, Asymptomatic Diseases, Spike Glycoprotein, Coronavirus, COVID-19, SARS-CoV-2
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	12 May 2021 10:04
Last Modified:	18 Jan 2023 22:47
DOI:	10.1016/j.xcrm.2021.100193
Open Access URL:	https://doi.org/10.1016/j.xcrm.2021.100193
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3122445