Differences in Tissue and Species Tropism of Reptarenavirus Species Studied by Vesicular Stomatitis Virus Pseudotypes



Korzyukov, Yegor, Iheozor-Ejiofor, Rommel, Levanov, Lev, Smura, Teemu, Hetzel, Udo, Szirovicza, Leonora, de la Torre, Juan Carlos, Martinez-Sobrido, Luis, Kipar, Anja ORCID: 0000-0001-7289-3459, Vapalahti, Olli
et al (show 1 more authors) (2020) Differences in Tissue and Species Tropism of Reptarenavirus Species Studied by Vesicular Stomatitis Virus Pseudotypes. VIRUSES-BASEL, 12 (4). E395-.

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Abstract

Reptarenaviruses cause Boid Inclusion Body Disease (BIBD), and co-infections by several reptarenaviruses are common in affected snakes. Reptarenaviruses have only been found in captive snakes, and their reservoir hosts remain unknown. In affected animals, reptarenaviruses appear to replicate in most cell types, but their complete host range, as well as tissue and cell tropism are unknown. As with other enveloped viruses, the glycoproteins (GPs) present on the virion's surface mediate reptarenavirus cell entry, and therefore, the GPs play a critical role in the virus cell and tissue tropism. Herein, we employed single cycle replication, GP deficient, recombinant vesicular stomatitis virus (VSV) expressing the enhanced green fluorescent protein (scrVSV∆G-eGFP) pseudotyped with different reptarenavirus GPs to study the virus cell tropism. We found that scrVSV∆G-eGFPs pseudotyped with reptarenavirus GPs readily entered mammalian cell lines, and some mammalian cell lines exhibited higher, compared to snake cell lines, susceptibility to reptarenavirus GP-mediated infection. Mammarenavirus GPs used as controls also mediated efficient entry into several snake cell lines. Our results confirm an important role of the virus surface GP in reptarenavirus cell tropism and that mamma-and reptarenaviruses exhibit high cross-species transmission potential.

Item Type: Article
Uncontrolled Keywords: BIBD, reptarenavirus, arenavirus, VSV, pseudotype, tissue tropism
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User: Symplectic Admin
Date Deposited: 19 May 2021 09:23
Last Modified: 18 Jan 2023 22:46
DOI: 10.3390/v12040395
Open Access URL: http://doi.org/10.3390/v12040395
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3123282