Mwangi, Peter N, Mogotsi, Milton T, Seheri, Mapaseka L, Mphahlele, M Jeffrey, Peenze, Ina, Esona, Mathew D, Kumwenda, Benjamin, Steele, A Duncan, Kirkwood, Carl D, Ndze, Valantine N et al (show 3 more authors)
(2020)
Whole Genome In-Silico Analysis of South African G1P[8] Rotavirus Strains before and after Vaccine Introduction over a Period of 14 Years.
VACCINES, 8 (4).
E609-.
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Whole Genome iIn-Silicoi Analysis of South African G1P[8] Rotavirus Strains Before and After Vaccine Introduction Over A Per.pdf - Published version Download (1MB) | Preview |
Abstract
Rotavirus G1P[8] strains account for more than half of the group A rotavirus (RVA) infections in children under five years of age, globally. A total of 103 stool samples previously characterized as G1P[8] and collected seven years before and seven years after introducing the Rotarix<sup>®</sup> vaccine in South Africa were processed for whole-genome sequencing. All the strains analyzed had a Wa-like constellation (G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1). South African pre- and post-vaccine G1 strains were clustered in G1 lineage-I and II while the majority (84.2%) of the P[8] strains were grouped in P[8] lineage-III. Several amino acid sites across ten gene segments with the exception of VP7 were under positive selective pressure. Except for the N147D substitution in the antigenic site of eight post-vaccine G1 strains when compared to both Rotarix<sup>®</sup> and pre-vaccine strains, most of the amino acid substitutions in the antigenic regions of post-vaccine G1P[8] strains were already present during the pre-vaccine period. Therefore, Rotarix<sup>®</sup> did not appear to have an impact on the amino acid differences in the antigenic regions of South African post-vaccine G1P[8] strains. However, continued whole-genome surveillance of RVA strains to decipher genetic changes in the post-vaccine period remains imperative.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | evolution, rotavirus strains, Wa-like constellation, whole-genome, lineages |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 17 Jun 2021 13:55 |
| Last Modified: | 13 Feb 2024 18:00 |
| DOI: | 10.3390/vaccines8040609 |
| Open Access URL: | https://www.mdpi.com/2076-393X/8/4/609 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3126740 |
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