How relevant are in vitro culture models for study of tick-pathogen interactions?

Salata, Cristiano, Moutailler, Sara, Attoui, Houssam, Zweygarth, Erich, Decker, Lygia and Bell-Sakyi, Lesley (2021) How relevant are in vitro culture models for study of tick-pathogen interactions? Pathogens and global health. 1 - 19.

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Abstract

Although tick-borne infectious diseases threaten human and animal health worldwide, with constantly increasing incidence, little knowledge is available regarding vector-pathogen interactions and pathogen transmission. In vivo laboratory study of these subjects using live, intact ticks is expensive, labor-intensive, and challenging from the points of view of biosafety and ethics. Several in vitro models have been developed, including over 70 continuous cell lines derived from multiple tick species and a variety of tick organ culture systems, facilitating many research activities. However, some limitations have to be considered in the translation of the results from the in vitro environment to the in vivo situation of live, intact ticks, and vertebrate hosts. In this review, we describe the available in vitro models and selected results from their application to the study of tick-borne viruses, bacteria, and protozoa, where possible comparing these results to studies in live, intact ticks. Finally, we highlight the strengths and weaknesses of in vitro tick culture models and their essential role in tick-borne pathogen research.

Item Type:	Article
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	02 Jul 2021 10:26
Last Modified:	18 Jan 2023 21:37
DOI:	10.1080/20477724.2021.1944539
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3128537