Al-Medhtiy, MH, Al-Salih, H, Almhanna, H and Al-Dabhawi, AH
(2020)
Investigation Study of Human Spermatozoa Proteome.
Indian Journal of Forensic Medicine & Toxicology, 14 (4).
pp. 2196-2202.
Abstract
The current study has been conducted on health human sperm to identify the different types of amino acids and entire proteins of sperm. Two methods have been applied to characterise of human sperm, firstly extract lysate of sperm, then digest and run it in mass spectrometry, secondly, total sperm lysate was run in SDS page gel and bands were stained with coomassie, then band was cut and digest to extract of whole amino acids and also was run in mass spectrometry. Result revealed that sperm have several proteins, which were involved with different binding proteins, soluble proteins, and glycoproteins, transmembrane proteins. Current result was displayed that mass spectrometry can be used to recognise data of proteins of sperm and detected types of it. This technique was applied on whole lysate and gel bands of sperm and created database of sperm proteins which can be investigated and consider more interesting for researchers. This study is concluded that human sperm have important transmembrane, enzymes, glycosylated proteins, glycoproteins and uncharacterised proteins which might be answered many theoretical aspects of biological and biochemical sperm migrations before and after fertilization.
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 12 Jul 2021 09:44 |
| Last Modified: | 18 Jan 2023 21:36 |
| DOI: | 10.37506/ijfmt.v14i4.11877 |
| Open Access URL: | https://doi.org/10.37506/ijfmt.v14i4.11877 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3129742 |
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