Physicochemical Characterisation of KEIF-The Intrinsically Disordered N-Terminal Region of Magnesium Transporter A



Jephthah, Stephanie, Mansson, Linda K, Belic, Domagoj ORCID: 0000-0002-7954-7046, Morth, Jens Preben and Skepo, Marie
(2020) Physicochemical Characterisation of KEIF-The Intrinsically Disordered N-Terminal Region of Magnesium Transporter A. BIOMOLECULES, 10 (4). E623-.

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Abstract

Magnesium transporter A (MgtA) is an active transporter responsible for importing magnesium ions into the cytoplasm of prokaryotic cells. This study focuses on the peptide corresponding to the intrinsically disordered N-terminal region of MgtA, referred to as KEIF. Primary-structure and bioinformatic analyses were performed, followed by studies of the undisturbed single chain using a combination of techniques including small-angle X-ray scattering, circular dichroism spectroscopy, and atomistic molecular-dynamics simulations. Moreover, interactions with large unilamellar vesicles were investigated by using dynamic light scattering, laser Doppler velocimetry, cryogenic transmission electron microscopy, and circular dichroism spectroscopy. KEIF was confirmed to be intrinsically disordered in aqueous solution, although extended and containing little β -structure and possibly PPII structure. An increase of helical content was observed in organic solvent, and a similar effect was also seen in aqueous solution containing anionic vesicles. Interactions of cationic KEIF with anionic vesicles led to the hypothesis that KEIF adsorbs to the vesicle surface through electrostatic and entropic driving forces. Considering this, there is a possibility that the biological role of KEIF is to anchor MgtA in the cell membrane, although further investigation is needed to confirm this hypothesis.

Item Type: Article
Uncontrolled Keywords: membrane proteins, intrinsically disordered proteins, circular dichroism spectroscopy, small-angle X-ray scattering, cryogenic transmission electron microscopy, molecular-dynamics simulations, protein-vesicle interactions, magnesium transporter, secondary structure
Divisions: Faculty of Science and Engineering > School of Physical Sciences
Depositing User: Symplectic Admin
Date Deposited: 09 Sep 2021 09:37
Last Modified: 18 Jan 2023 21:29
DOI: 10.3390/biom10040623
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3136486