Incorporation of Functional Rubisco Activases into Engineered Carboxysomes to Enhance Carbon Fixation.

Chen, Taiyu, Fang, Yi, Jiang, Qiuyao, Dykes, Gregory F, Lin, Yongjun ORCID: 0000-0002-3818-4816, Price, G Dean, Long, Benedict M ORCID: 0000-0002-4616-2967 and Liu, Lu-Ning (2021) Incorporation of Functional Rubisco Activases into Engineered Carboxysomes to Enhance Carbon Fixation. ACS synthetic biology.

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Abstract

The carboxysome is a versatile paradigm of prokaryotic organelles and is a proteinaceous self-assembling microcompartment that plays essential roles in carbon fixation in all cyanobacteria and some chemoautotrophs. The carboxysome encapsulates the central CO₂-fixing enzyme, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), using a polyhedral protein shell that is selectively permeable to specific metabolites in favor of Rubisco carboxylation. There is tremendous interest in repurposing carboxysomes to boost carbon fixation in heterologous organisms. Here, we develop the design and engineering of α-carboxysomes by coexpressing the Rubisco activase components CbbQ and CbbO with α-carboxysomes in <i>Escherichia coli</i>. Our results show that CbbQ and CbbO could assemble into the reconstituted α-carboxysome as intrinsic components. Incorporation of both CbbQ and CbbO within the carboxysome promotes activation of Rubisco and enhances the CO₂-fixation activities of recombinant carboxysomes. We also show that the structural composition of these carboxysomes could be modified in different expression systems, representing the plasticity of the carboxysome architecture. In translational terms, our study informs strategies for engineering and modulating carboxysomes in diverse biotechnological applications.

Item Type:	Article
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	20 Oct 2021 08:42
Last Modified:	18 Jan 2023 21:26
DOI:	10.1021/acssynbio.1c00311
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3140976

Available Versions of this Item

• Incorporation of Functional Rubisco Activases into Engineered Carboxysomes to Enhance Carbon Fixation. (deposited 20 Oct 2021 08:42) [Currently Displayed]
  • Incorporation of Functional Rubisco Activases into Engineered Carboxysomes to Enhance Carbon Fixation. (deposited 21 Jan 2022 15:49)