Shrwani, Khalid, Sharma, Ravi, Krishnan, Madhan, Jones, Terry, Mayora-Neto, Martin, Cantoni, Diego, Temperton, Nigel J, Dobson, Susan L 
ORCID: 0000-0002-4958-0021, Subramaniam, Krishanthi ORCID: 0000-0002-1734-9351, McNamara, Paul S ORCID: 0000-0002-7055-6034 et al (show 3 more authors)
(2021)
Detection of Serum Cross-Reactive Antibodies and Memory Response to SARS-CoV-2 in Prepandemic and Post-COVID-19 Convalescent Samples.
JOURNAL OF INFECTIOUS DISEASES, 224 (8).
pp. 1305-1315.
Abstract
<h4>Background</h4>A notable feature of coronavirus disease 2019 (COVID-19) is that children are less susceptible to severe disease. Children are known to experience more infections with endemic human coronaviruses (HCoVs) compared to adults. Little is known whether HCoV infections lead to cross-reactive anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies.<h4>Methods</h4>We investigated the presence of cross-reactive anti-SARS-CoV-2 IgG antibodies to spike 1 (S1), S1-receptor-binding domain (S1-RBD), and nucleocapsid protein (NP) by enzyme-linked immunosorbent assays, and neutralizing activity by a SARS-CoV-2 pseudotyped virus neutralization assay, in prepandemic sera collected from children (n = 50) and adults (n = 45), and compared with serum samples from convalescent COVID-19 patients (n = 16).<h4>Results</h4>A significant proportion of children (up to 40%) had detectable cross-reactive antibodies to SARS-CoV-2 S1, S1-RBD, and NP antigens, and the anti-S1 and anti-S1-RBD antibody levels correlated with anti-HCoV-HKU1 and anti-HCoV-OC43 S1 antibody titers in prepandemic samples (P < .001). There were marked increases of anti-HCoV-HKU1 and - OC43 S1 (but not anti-NL63 and -229E S1-RBD) antibody titers in serum samples from convalescent COVID-19 patients (P < .001), indicating an activation of cross-reactive immunological memory to β-coronavirus spike.<h4>Conclusions</h4>We demonstrated cross-reactive anti-SARS-CoV-2 antibodies in prepandemic serum samples from children and young adults. Promoting this cross-reactive immunity and memory response derived from common HCoV may be an effective strategy against SARS-COV-2 and future novel coronaviruses.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COVID-19, SARS-CoV-2, common human coronavirus (HCoV)-HKU1, HCoV-NL63, serum antibody, cross-reactive immunity, immunological memory |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 28 Oct 2021 10:11 |
| Last Modified: | 18 Jan 2023 21:25 |
| DOI: | 10.1093/infdis/jiab333 |
| Open Access URL: | https://doi.org/10.1093/infdis/jiab333 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3141938 |
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