Hyperintensity of integrin-targeted fluorescence agent IntegriSense750 accurately predicts flap necrosis compared to Indocyanine green

Hicks, Melanie D, Ovaitt, Alyssa K, Fleming, Jason C ORCID: 0000-0001-7963-1224, Sorace, Anna G, Song, Patrick N, Mansur, Ameer, Bs, Yolanda E Hartman, Rosenthal, Eben L, Warram, Jason M and Thomas, Carissa M (2022) Hyperintensity of integrin-targeted fluorescence agent IntegriSense750 accurately predicts flap necrosis compared to Indocyanine green. HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, 44 (1). pp. 134-142.

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Official URL: http://dx.doi.org/10.1002/hed.26914

Abstract

<h4>Background</h4>Flap necrosis is a feared complication of reconstructive surgery. Current methods of prediction using Indocyanine green (ICG) lack specificity. IntegriSense750 is a fluorescence agent that binds sites of vascular remodeling. We hypothesized that IntegriSense750 better predicts flap compromise compared to ICG.<h4>Methods</h4>Fifteen mice underwent lateral thoracic artery axial flap harvest. Mice received an injection of ICG (n = 7) or IntegriSense750 (n = 8) daily from postoperative days (POD) 0-3 and were imaged daily. Mean signal-to-background ratios quantified the change in fluorescence as necrosis progressed.<h4>Results</h4>Mean signal-to-background ratio was significantly higher for IntegriSense750 compared to ICG on POD0 (1.47 ± 0.17 vs. 0.86 ± 0.21, p = 0.01) and daily through POD3 (2.12 ± 0.70 vs. 0.96 ± 0.29, p < 0.001).<h4>Conclusions</h4>IntegriSense750 demonstrates increased signal-to-background ratio at areas of flap distress compared to ICG which may increase identification of flap necrosis and improve patient outcomes.

Item Type:	Article
Uncontrolled Keywords:	axial pattern flap, flap necrosis, Indocyanine green, IntegriSense750, murine flap model
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	10 Mar 2023 09:56
Last Modified:	10 Mar 2023 09:56
DOI:	10.1002/hed.26914
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3142742