de, Silva Thushan I, Liu, Guihai, Lindsey, Benjamin B, Dong, Danning, Moore, Shona C 
ORCID: 0000-0001-8610-2806, Hsu, Nienyun Sharon, Shah, Dhruv, Wellington, Dannielle, Mentzer, Alexander J, Angyal, Adrienn et al (show 12 more authors)
(2021)
The impact of viral mutations on recognition by SARS-CoV-2 specific T cells.
ISCIENCE, 24 (11).
103353-.
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Abstract
We identify amino acid variants within dominant SARS-CoV-2 T cell epitopes by interrogating global sequence data. Several variants within nucleocapsid and ORF3a epitopes have arisen independently in multiple lineages and result in loss of recognition by epitope-specific T cells assessed by IFN-γ and cytotoxic killing assays. Complete loss of T cell responsiveness was seen due to Q213K in the A∗01:01-restricted CD8+ ORF3a epitope FTSDYYQLY<sub>207-215</sub>; due to P13L, P13S, and P13T in the B∗27:05-restricted CD8+ nucleocapsid epitope QRNAPRITF<sub>9-17</sub>; and due to T362I and P365S in the A∗03:01/A∗11:01-restricted CD8+ nucleocapsid epitope KTFPPTEPK<sub>361-369</sub>. CD8+ T cell lines unable to recognize variant epitopes have diverse T cell receptor repertoires. These data demonstrate the potential for T cell evasion and highlight the need for ongoing surveillance for variants capable of escaping T cell as well as humoral immunity.
| Item Type: | Article |
|---|---|
| Additional Information: | Source info: CELL-REPORTS-D-21-02019 |
| Uncontrolled Keywords: | COVID-19 Genomics UK (COG-UK) Consortium, ISARIC4C Investigators |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 18 Nov 2021 15:07 |
| Last Modified: | 18 Jan 2023 21:24 |
| DOI: | 10.1016/j.isci.2021.103353 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3143428 |
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