Interstrain Variability of Human Vaginal Lactobacillus crispatus for Metabolism of Biogenic Amines and Antimicrobial Activity against Urogenital Pathogens.

Puebla-Barragan, Scarlett, Watson, Emiley, van der Veer, Charlotte ORCID: 0000-0002-1685-1622, Chmiel, John A, Carr, Charles, Burton, Jeremy P, Sumarah, Mark, Kort, Remco and Reid, Gregor (2021) Interstrain Variability of Human Vaginal Lactobacillus crispatus for Metabolism of Biogenic Amines and Antimicrobial Activity against Urogenital Pathogens. Molecules (Basel, Switzerland), 26 (15). 4538-.

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Official URL: http://dx.doi.org/10.3390/molecules26154538

Abstract

Lactobacillus crispatus is the dominant species in the vagina of many women. With the potential for strains of this species to be used as a probiotic to help prevent and treat dysbiosis, we investigated isolates from vaginal swabs with Lactobacillus-dominated and a dysbiotic microbiota. A comparative genome analysis led to the identification of metabolic pathways for synthesis and degradation of three major biogenic amines in most strains. However, targeted metabolomic analysis of the production and degradation of biogenic amines showed that certain strains have either the ability to produce or to degrade these compounds. Notably, six strains produced cadaverine, one produced putrescine, and two produced tyramine. These biogenic amines are known to raise vaginal pH, cause malodour, and make the environment more favourable to vaginal pathogens. In vitro experiments confirmed that strains isolated from women with a dysbiotic vaginal microbiota have higher antimicrobial effects against the common urogenital pathogens Escherichia coli and Enterococcus faecium. The results indicate that not all L. crispatus vaginal strains appear suitable for probiotic application and the basis for selection should not be only the overall composition of the vaginal microbiota of the host from which they came, but specific biochemical and genetic traits.

Item Type:	Article
Uncontrolled Keywords:	Vagina, Humans, Prevotella, Escherichia coli, Enterococcus faecium, Candida albicans, Biogenic Amines, Anti-Infective Agents, Genomics, Phylogeny, Probiotics, Female, Female Urogenital Diseases, Metabolomics, Metabolome, Microbiota, Dysbiosis, Lactobacillus crispatus
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Depositing User:	Symplectic Admin
Date Deposited:	09 Dec 2021 10:16
Last Modified:	18 Jan 2023 21:23
DOI:	10.3390/molecules26154538
Open Access URL:	https://doi.org/10.3390/molecules26154538
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3145050