CyTOF mass cytometry reveals phenotypically distinct human blood neutrophil populations differentially correlated with melanoma stage

Zhu, Yanfang Peipei, Eggert, Tobias, Araujo, Daniel J, Vijayanand, Pandurangan, Ottensmeier, Christian Hermann ORCID: 0000-0003-3619-1657 and Hedrick, Catherine C (2020) CyTOF mass cytometry reveals phenotypically distinct human blood neutrophil populations differentially correlated with melanoma stage. JOURNAL FOR IMMUNOTHERAPY OF CANCER, 8 (2). e000473-.

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Official URL: http://dx.doi.org/10.1136/jitc-2019-000473

Abstract

<h4>Background</h4>Understanding neutrophil heterogeneity and its relationship to disease progression has become a recent focus of cancer research. Indeed, several studies have identified neutrophil subpopulations associated with protumoral or antitumoral functions. However, this work has been hindered by a lack of widely accepted markers with which to define neutrophil subpopulations.<h4>Methods</h4>To identify markers of neutrophil heterogeneity in cancer, we used single-cell cytometry by time-of-flight (CyTOF) coupled with high-dimensional analysis on blood samples from treatment-naïve patients with melanoma.<h4>Results</h4>Our efforts allowed us to identify seven blood neutrophil clusters, including two previously identified individual populations. Interrogation of these neutrophil subpopulations revealed a positive trend between specific clusters and disease stage. Finally, we recapitulated these seven blood neutrophil populations via flow cytometry and found that they exhibited diverse capacities for phagocytosis and reactive oxygen species production in vitro.<h4>Conclusions</h4>Our data provide a refined consensus on neutrophil heterogeneity markers, enabling a prospective functional evaluation in patients with solid tumors.

Item Type:	Article
Uncontrolled Keywords:	haematology, immunology
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	22 Dec 2021 09:21
Last Modified:	09 Feb 2024 17:09
DOI:	10.1136/jitc-2019-000473
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3145814