ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the delineation of HS biosynthesis

Meneghetti, Maria CZ, Deboni, Paula, Palomino, Carlos MV, Braga, Luiz P, Cavalheiro, Renan P, Viana, Gustavo M, Yates, Edwin A ORCID: 0000-0001-9365-5433, Nader, Helena B and Lima, Marcelo A (2021) ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the delineation of HS biosynthesis. CARBOHYDRATE POLYMERS, 255. 117477-.

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Official URL: http://dx.doi.org/10.1016/j.carbpol.2020.117477

Abstract

The cell surface and extracellular matrix polysaccharide, heparan sulfate (HS) conveys chemical information to control crucial biological processes. HS chains are synthesized in a non-template driven process mainly in the Golgi apparatus, involving a large number of enzymes capable of subtly modifying its substitution pattern, hence, its interactions and biological effects. Changes in the localization of HS-modifying enzymes throughout the Golgi were found to correlate with changes in the structure of HS, rather than protein expression levels. Following BFA treatment, the HS-modifying enzymes localized preferentially in COPII vesicles and at the trans-Golgi. Shortly after heparin treatment, the HS-modifying enzyme moved from cis to trans-Golgi, which coincided with increased HS sulfation. Finally, it was shown that COPI subunits and Sec24 gene expression changed. Collectively, these findings demonstrate that knowledge of the ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the complete delineation of HS biosynthesis.

Item Type:	Article
Uncontrolled Keywords:	Biosynthesis, Heparan sulfate, COPI, COPII, Golgi apparatus
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	23 Dec 2021 08:57
Last Modified:	18 Jan 2023 21:18
DOI:	10.1016/j.carbpol.2020.117477
Open Access URL:	https://doi.org/10.1016/j.carbpol.2020.117477
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3145944