Bothwell, Steven W, Omileke, Daniel, Hood, Rebecca J, Pepperall, Debbie-Gai, Azarpeykan, Sara, Patabendige, Adjanie 
ORCID: 0000-0003-1550-3084 and Spratt, Neil J
(2021)
Altered Cerebrospinal Fluid Clearance and Increased Intracranial Pressure in Rats 18 h After Experimental Cortical Ischaemia.
FRONTIERS IN MOLECULAR NEUROSCIENCE, 14.
712779-.
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Altered Cerebrospinal Fluid Clearance and Increased Intracranial Pressure in Rats 18 h After Experimental Cortical Ischaemia.pdf - Published version Download (1MB) | Preview |
Abstract
Oedema-independent intracranial pressure (ICP) rise peaks 20-22-h post-stroke in rats and may explain early neurological deterioration. Cerebrospinal fluid (CSF) volume changes may be involved. Cranial CSF clearance primarily occurs <i>via</i> the cervical lymphatics and movement into the spinal portion of the cranio-spinal compartment. We explored whether impaired CSF clearance at these sites could explain ICP rise after stroke. We recorded ICP at baseline and 18-h post-stroke, when we expect changes contributing to peak ICP to be present. CSF clearance was assessed in rats receiving photothrombotic stroke or sham surgery by intraventricular tracer infusion. Tracer concentration was quantified in the deep cervical lymph nodes <i>ex vivo</i> and tracer transit to the spinal subarachnoid space was imaged <i>in vivo</i>. ICP rose significantly from baseline to 18-h post-stroke in stroke vs. sham rats [median = 5 mmHg, interquartile range (IQR) = 0.1-9.43, <i>n</i> = 12, vs. -0.3 mmHg, IQR = -1.9-1.7, <i>n</i> = 10], <i>p</i> = 0.03. There was a bimodal distribution of rats with and without ICP rise. Tracer in the deep cervical lymph nodes was significantly lower in stroke with ICP rise (0 μg/mL, IQR = 0-0.11) and without ICP rise (0 μg/mL, IQR = 0-4.47) compared with sham rats (4.17 μg/mL, IQR = 0.74-8.51), <i>p</i> = 0.02. ICP rise was inversely correlated with faster CSF transit to the spinal subarachnoid space (<i>R</i> = -0.59, <i>p</i> = 0.006, Spearman's correlation). These data suggest that reduced cranial clearance of CSF via cervical lymphatics may contribute to post-stroke ICP rise, partially compensated <i>via</i> increased spinal CSF outflow.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | cerebrospinal fluid, infarct expansion, intracranial pressure, ischaemia, lymphatics, neurological deterioration, spinal clearance, stroke |
| Divisions: | Faculty of Health and Life Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 21 Jan 2022 15:56 |
| Last Modified: | 18 Jan 2023 21:15 |
| DOI: | 10.3389/fnmol.2021.712779 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3147352 |
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