CSF Secretion Is Not Altered by NKCC1 Nor TRPV4 Antagonism in Healthy Rats

Bothwell, Steven W, Omileke, Daniel, Patabendige, Adjanie ORCID: 0000-0003-1550-3084 and Spratt, Neil J (2021) CSF Secretion Is Not Altered by NKCC1 Nor TRPV4 Antagonism in Healthy Rats. BRAIN SCIENCES, 11 (9). 1117-.

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Official URL: http://dx.doi.org/10.3390/brainsci11091117

Abstract

<h4>Background</h4>Cerebrospinal fluid (CSF) secretion can be targeted to reduce elevated intracranial pressure (ICP). Sodium-potassium-chloride cotransporter 1 (NKCC1) antagonism is used clinically. However, supporting evidence is limited. The transient receptor potential vanilloid-4 (TRPV4) channel may also regulate CSF secretion and ICP elevation. We investigated whether antagonism of these proteins reduces CSF secretion.<h4>Methods</h4>We quantified CSF secretion rates in male Wistar rats. The cerebral aqueduct was blocked with viscous mineral oil, and a lateral ventricle was cannulated. Secretion rate was measured at baseline and after antagonist administration. Acetazolamide was administered as a positive control to confirm changes in CSF secretion rates.<h4>Results</h4>Neither NKCC1, nor TRPV4 antagonism altered CSF secretion rate from baseline, <i>n</i> = 3, t(2) = 1.14, <i>p</i> = 0.37, and <i>n</i> = 4, t(3) = 0.58, <i>p</i> = 0.6, respectively. Acetazolamide reduced CSF secretion by ~50% across all groups, <i>n</i> = 7, t(6) = 4.294, <i>p</i> = 0.005.<h4>Conclusions</h4>Acute antagonism of NKCC1 and TRPV4 proteins at the choroid plexus does not reduce CSF secretion in healthy rats. Further investigation of protein changes and antagonism should be explored in neurological disease where increased CSF secretion and ICP are observed before discounting the therapeutic potential of protein antagonism at these sites.

Item Type:	Article
Uncontrolled Keywords:	cerebrospinal fluid, choroid plexus, NKCC1, TRPV4, intracranial pressure, loop diuretics
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	27 Jan 2022 15:08
Last Modified:	18 Jan 2023 21:14
DOI:	10.3390/brainsci11091117
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3147715