Wolput, Sanne, Makumi, Angela, Wicke, Laura, Baecker, Leonard E, Cenens, William, Briers, Yves, Wenner, Nicolas A, Owen, Sian V, Hinton, Jay CD, Lavigne, Rob et al (show 1 more authors)
(2022)
Transcriptional Organization of the <i>Salmonella</i> Typhimurium Phage P22 <i>pid</i> ORFan Locus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23 (3).
1253-.
Abstract
Many phage genes lack sequence similarity to any other open reading frame (ORF) in current databases. These enigmatic ORFan genes can have a tremendous impact on phage propagation and host interactions but often remain experimentally unexplored. We previously revealed a novel interaction between phage P22 and its <i>Salmonella</i> Typhimurium host, instigated by the ORFan gene <i>pid</i> (for phage P22 encoded instigator of <i>dgo</i> expression) and resulting in derepression of the host <i>dgoRKAT</i> operon. The <i>pid</i> gene is highly expressed in phage carrier cells that harbor a polarly located P22 episome that segregates asymmetrically among daughter cells. Here, we discovered that the <i>pid</i> locus is fitted with a weak promoter, has an exceptionally long 5' untranslated region that is instructive for a secondary <i>pid</i> mRNA species, and has a 3' Rho-independent termination loop that is responsible for stability of the <i>pid</i> transcript.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | phage-host interactions, Salmonella Typhimurium, phage P22, pid ORFan locus, dgoRKAT operon |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 31 Jan 2022 16:45 |
| Last Modified: | 24 Jan 2024 17:54 |
| DOI: | 10.3390/ijms23031253 |
| Open Access URL: | https://www.mdpi.com/1422-0067/23/3/1253/htm |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3147888 |
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