Hernández-Triana, Luis M 
ORCID: 0000-0001-7058-8848, Folly, Arran J ORCID: 0000-0003-0106-2185, Barrero, Elsa, Lumley, Sarah ORCID: 0000-0002-8605-3650, Del Mar Fernández de Marco, Maria, Sewgobind, Sanam, McElhinney, Lorraine M ORCID: 0000-0002-6022-348X, Fooks, Anthony R and Johnson, Nicholas
(2021)
Oral susceptibility of aedine and culicine mosquitoes (Diptera: Culicidae) to Batai Orthobunyavirus.
Parasites & vectors, 14 (1).
566-.
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Oral susceptibility of aedine and culicine mosquitoes (Diptera Culicidae) to Batai Orthobunyavirus.pdf - Published version Download (906kB) | Preview |
Abstract
<h4>Background</h4>A number of zoonotic mosquito-borne viruses have emerged in Europe in recent decades. Batai virus (BATV), a member of the genus Orthobunyavirus, is one example of a relatively newly emerged mosquito-borne virus, having been detected in mosquitoes and livestock. We conducted vector competency studies on three mosquito species at a low temperature to assess whether Aedes and Culex mosquito species are susceptible to infection with BATV.<h4>Methods</h4>Colonised lines of Aedes aegypti and Culex pipiens and a wild-caught species, Aedes detritus, were orally inoculated with BATV strain 53.2, originally isolated from mosquitoes trapped in Germany in 2009. Groups of blood-fed female mosquitoes were maintained at 20 °C for 7 or 14 days. Individual mosquitoes were screened for the presence of BATV in body, leg and saliva samples for evidence of infection, dissemination and transmission, respectively. BATV RNA was detected by reverse transcription-PCR, and positive results confirmed by virus isolation in Vero cells.<h4>Results</h4>Aedes detritus was highly susceptible to BATV, with an infection prevalence of ≥ 80% at both measurement time points. Disseminated infections were recorded in 30.7-41.6% of Ae. detritus, and evidence of virus transmission with BATV in saliva samples (n = 1, days post-infection: 14) was observed. Relatively lower rates of infection for Ae. aegypti and Cx. pipiens were observed, with no evidence of virus dissemination or transmission at either time point.<h4>Conclusions</h4>This study shows that Ae. detritus may be a competent vector for BATV at 20 °C, whereas Ae. aegypti and Cx. pipiens were not competent. Critically, the extrinsic incubation period appears to be ≤ 7 days for Ae. detritus, which may increase the onward transmissibility potential of BATV in these populations.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Saliva, Animals, Humans, Culicidae, Bunyamwera virus, Bunyaviridae Infections, Europe, Female, Male, Mosquito Vectors |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 08 Feb 2022 09:25 |
| Last Modified: | 18 Jan 2023 21:13 |
| DOI: | 10.1186/s13071-021-05070-0 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3148486 |
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