Immunohistochemical Markers of Apoptotic and Hypoxic Damage Facilitate Evidence-Based Assessment in Pups with Neurological Disorders

Orekhova, Ksenia, Mazzariol, Sandro, Sussan, Beatrice, Bucci, Massimo, Bonsembiante, Federico, Verin, Ranieri ORCID: 0000-0001-9366-5682 and Centelleghe, Cinzia (2021) Immunohistochemical Markers of Apoptotic and Hypoxic Damage Facilitate Evidence-Based Assessment in Pups with Neurological Disorders. VETERINARY SCIENCES, 8 (10). 203-.

Text
Immunohistochemical Markers of Apoptotic and Hypoxic Damage Facilitate Evidence-Based Assessment in Pups with Neurological D.pdf - Published version
Download (1MB) | Preview

Official URL: http://dx.doi.org/10.3390/vetsci8100203

Abstract

Seizures in puppies often present a diagnostic challenge in terms of identifying and treating the underlying cause. Dog breeds with mutations of the <i>MDR1</i>-gene are known to show adverse reactions to certain drugs, yet metabolic imbalance exacerbated by physiologically immature organs and other contributing pathologies require consideration before arriving at a diagnosis. This study analysed the brains of two male, 5-week-old Australian Shepherd siblings that died after displaying severe neurological symptoms upon administration of MilproVet<sup>®</sup> to treat severe intestinal helminth infection. Despite the initial symptoms being similar, their case histories varied in terms of the symptom duration, access to supportive therapy and post-mortem interval. Histopathology and immunohistochemistry were used to obtain more information about the phase of the pathological processes in the brain, employing protein markers associated with acute hypoxic damage (β-amyloid precursor protein/APP) and apoptosis (diacylglycerolkinase-ζ/DGK-ζ, apoptotic protease activating factor 1/Apaf1, and B-cell lymphoma related protein 2/Bcl-2). The results seem to reflect the course of the animals' clinical deterioration, implicating that the hypoxic damage to the brains was incompatible with life, and suggesting the usefulness of the mentioned immunohistochemical markers in clarifying the cause of death in animals with acute neurological deficits.

Item Type:	Article
Uncontrolled Keywords:	seizures, hypoxic brain injury, canine diacylglycerolkinase zeta, beta-amyloid precursor protein, Apaf1 protein, Bcl-2 protein, MDR1 mutation
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	08 Feb 2022 14:20
Last Modified:	03 Feb 2024 09:54
DOI:	10.3390/vetsci8100203
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3148523