How relevant are in vitro culture models for study of tick-pathogen interactions?

Salata, Cristiano, Moutailler, Sara, Attoui, Houssam, Zweygarth, Erich, Decker, Lygia and Bell-Sakyi, Lesley ORCID: 0000-0002-7305-0477 (2021) How relevant are in vitro culture models for study of tick-pathogen interactions? Pathogens and global health, 115 (7-8). pp. 1-19.

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Abstract

Although tick-borne infectious diseases threaten human and animal health worldwide, with constantly increasing incidence, little knowledge is available regarding vector-pathogen interactions and pathogen transmission. <i>In vivo</i> laboratory study of these subjects using live, intact ticks is expensive, labor-intensive, and challenging from the points of view of biosafety and ethics. Several <i>in vitro</i> models have been developed, including over 70 continuous cell lines derived from multiple tick species and a variety of tick organ culture systems, facilitating many research activities. However, some limitations have to be considered in the translation of the results from the <i>in vitro</i> environment to the <i>in vivo</i> situation of live, intact ticks, and vertebrate hosts. In this review, we describe the available <i>in vitro</i> models and selected results from their application to the study of tick-borne viruses, bacteria, and protozoa, where possible comparing these results to studies in live, intact ticks. Finally, we highlight the strengths and weaknesses of <i>in vitro</i> tick culture models and their essential role in tick-borne pathogen research.

Item Type:	Article
Uncontrolled Keywords:	Ticks, tick cell lines, organ culture, tick-borne, in vitro model, virus, bacteria, protozoa
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	18 Feb 2022 09:03
Last Modified:	18 Jan 2023 21:12
DOI:	10.1080/20477724.2021.1944539
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3149131

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• How relevant are in vitro culture models for study of tick-pathogen interactions? (deposited 02 Jul 2021 10:26)
  • How relevant are in vitro culture models for study of tick-pathogen interactions? (deposited 18 Feb 2022 09:03) [Currently Displayed]