Recognition Pattern of the Fasciola hepatica Excretome/Secretome during the Course of an Experimental Infection in Sheep by 2D Immunoproteomics.

Becerro-Recio, David ORCID: 0000-0001-8876-2592, González-Miguel, Javier ORCID: 0000-0003-4279-4761, Ucero, Alberto, Sotillo, Javier ORCID: 0000-0002-1443-7233, Martínez-Moreno, Álvaro ORCID: 0000-0003-4298-6894, Pérez-Arévalo, José, Cwiklinski, Krystyna ORCID: 0000-0001-5577-2735, Dalton, John P and Siles-Lucas, Mar (2021) Recognition Pattern of the Fasciola hepatica Excretome/Secretome during the Course of an Experimental Infection in Sheep by 2D Immunoproteomics. Pathogens (Basel, Switzerland), 10 (6). 725-.

Access the full-text of this item by clicking on the Open Access link.

Official URL: http://dx.doi.org/10.3390/pathogens10060725

Abstract

Excretory/secretory products released by helminth parasites have been widely studied for their diagnostic utility, immunomodulatory properties, as well as for their use as vaccines. Due to their location at the host/parasite interface, the characterization of parasite secretions is important to unravel the molecular interactions governing the relationships between helminth parasites and their hosts. In this study, the excretory/secretory products from adult worms of the trematode Fasciola hepatica (FhES) were employed in a combination of two-dimensional electrophoresis, immunoblot and mass spectrometry, to analyze the immune response elicited in sheep during the course of an experimental infection. Ten different immunogenic proteins from FhES recognized by serum samples from infected sheep at 4, 8, and/or 12 weeks post-infection were identified. Among these, different isoforms of cathepsin L and B, peroxiredoxin, calmodulin, or glutathione S-transferase were recognized from the beginning to the end of the experimental infection, suggesting their potential role as immunomodulatory antigens. Furthermore, four FhES proteins (C2H2-type domain-containing protein, ferritin, superoxide dismutase, and globin-3) were identified for the first time as non-immunogenic proteins. These results may help to further understand host/parasite relationships in fasciolosis, and to identify potential diagnostic molecules and drug target candidates of F. hepatica.

Item Type:	Article
Uncontrolled Keywords:	Fasciola hepatica, excretory/secretory antigens, immunoproteomics, sheep
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	24 Feb 2022 09:36
Last Modified:	14 Mar 2024 17:46
DOI:	10.3390/pathogens10060725
Open Access URL:	https://www.mdpi.com/2076-0817/10/6/725
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3149567