Saithong, Supichcha, Worasilchai, Navaporn, Saisorn, Wilasinee 
ORCID: 0000-0002-0074-0389, Udompornpitak, Kanyarat ORCID: 0000-0002-0928-1801, Bhunyakarnjanarat, Thansita, Chindamporn, Ariya, Tovichayathamrong, Punyot, Torvorapanit, Pattama ORCID: 0000-0001-9507-6339, Chiewchengchol, Direkrit ORCID: 0000-0003-1824-7012, Chancharoenthana, Wiwat ORCID: 0000-0002-2965-146X et al (show 1 more authors)
(2022)
Neutrophil Extracellular Traps in Severe SARS-CoV-2 Infection: A Possible Impact of LPS and (1→3)-β-D-glucan in Blood from Gut Translocation.
Cells, 11 (7).
1103-.
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Neutrophil Extracellular Traps in Severe SARS-CoV-2 Infection A Possible Impact of LPS and (1→3)-β-D-glucan in Blood from Gu.pdf - Published version Download (3MB) | Preview |
Abstract
Due to limited data on the link between gut barrier defects (leaky gut) and neutrophil extracellular traps (NETs) in coronavirus disease 2019 (COVID-19), blood samples of COVID-19 cases-mild (upper respiratory tract symptoms without pneumonia; <i>n</i> = 27), moderate (pneumonia without hypoxia; <i>n</i> = 28), and severe (pneumonia with hypoxia; <i>n</i> = 20)-versus healthy control (<i>n</i> = 15) were evaluated, together with in vitro experiments. Accordingly, neutrophil counts, serum cytokines (IL-6 and IL-8), lipopolysaccharide (LPS), bacteria-free DNA, and NETs parameters (fluorescent-stained nuclear morphology, dsDNA, neutrophil elastase, histone-DNA complex, and myeloperoxidase-DNA complex) were found to differentiate COVID-19 severity, whereas serum (1→3)-β-D-glucan (BG) was different between the control and COVID-19 cases. Despite non-detectable bacteria-free DNA in the blood of healthy volunteers, using blood bacteriome analysis, proteobacterial DNA was similarly predominant in both control and COVID-19 cases (all severities). In parallel, only COVID-19 samples from moderate and severe cases, but not mild cases, were activated in vitro NETs, as determined by supernatant dsDNA, <i>Peptidyl Arginine Deiminase 4</i>, and nuclear morphology. With neutrophil experiments, LPS plus BG (LPS + BG) more prominently induced NETs, cytokines, <i>NFκB</i>, and reactive oxygen species, when compared with the activation by each molecule alone. In conclusion, pathogen molecules (LPS and BG) from gut translocation along with neutrophilia and cytokinemia in COVID-19-activated, NETs-induced hyperinflammation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Humans, Pneumonia, Lipopolysaccharides, beta-Glucans, Cytokines, Extracellular Traps, Hypoxia, COVID-19, SARS-CoV-2 |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 17 May 2022 15:44 |
| Last Modified: | 18 Jan 2023 21:01 |
| DOI: | 10.3390/cells11071103 |
| Open Access URL: | https://www.mdpi.com/2073-4409/11/7/1103 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3155001 |
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