The Two-Way Interaction between the Molecules That Cause Vaginal Malodour and Lactobacilli: An Opportunity for Probiotics.



Puebla-Barragan, Scarlett ORCID: 0000-0002-0276-3373, Akouris, Polycronis Paul ORCID: 0000-0002-3214-6130, Al, Kait F ORCID: 0000-0002-5905-6581, Carr, Charles ORCID: 0000-0002-1877-160X, Lamb, Britney, Sumarah, Mark ORCID: 0000-0003-0977-1656, van der Veer, Charlotte ORCID: 0000-0002-1685-1622, Kort, Remco ORCID: 0000-0003-3674-598X, Burton, Jeremy ORCID: 0000-0002-3591-6436 and Reid, Gregor ORCID: 0000-0001-9658-5696
(2021) The Two-Way Interaction between the Molecules That Cause Vaginal Malodour and Lactobacilli: An Opportunity for Probiotics. International journal of molecular sciences, 22 (22). 12279-.

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Abstract

Vaginal malodour is a sign of dysbiosis. The biogenic amines (BAs) cadaverine, putrescine and tyramine are known to be causative compounds. Recent reports suggest these compounds produced by pathogens might have a role beyond causing malodour; namely inhibiting the growth of lactobacilli bacteria that are crucial in the maintenance of vaginal homeostasis. The aim of this study was to identify whether certain lactobacilli strains could reduce BAs and to evaluate how <i>Lactobacillus</i> species were affected by these compounds. Using LC-MS and HPLC-UV, five <i>Lactobacillus crispatus</i> strains were identified as being capable of significantly reducing BAs from the media under in vitro conditions. Through 16S rRNA gene sequencing of vaginal swabs exposed to Bas, cadaverine was found to reduce the relative abundance of lactobacilli. When <i>L. crispatus</i> was exposed to media supplemented with BAs with an HCl adjusted lower pH, its growth was enhanced, demonstrating the relevance of the maintenance of an acidic vaginal environment. If strains are to be developed for probiotic application to alleviate bacterial vaginosis and other conditions affecting large numbers of women worldwide, their ability to adapt to Bas and regulate pH should be part of the experimentation.

Item Type: Article
Uncontrolled Keywords: Vagina, Humans, Lactobacillus, Vaginosis, Bacterial, Probiotics, Female, Dysbiosis
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Depositing User: Symplectic Admin
Date Deposited: 20 May 2022 13:34
Last Modified: 10 Feb 2024 02:59
DOI: 10.3390/ijms222212279
Open Access URL: https://www.mdpi.com/1422-0067/22/22/12279
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3155219