New Cell Lines Derived from European Tick Species

Bell-Sakyi, Lesley ORCID: 0000-0002-7305-0477, Hartley, Catherine S, Khoo, Jing Jing ORCID: 0000-0003-1285-6052, Forth, Jan Hendrik, Palomar, Ana M and Makepeace, Benjamin L ORCID: 0000-0002-6100-6727 (2022) New Cell Lines Derived from European Tick Species. MICROORGANISMS, 10 (6). 1086-.

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Official URL: http://dx.doi.org/10.3390/microorganisms10061086

Abstract

Tick cell lines are important tools for research on ticks and the pathogens they transmit. Here, we report the establishment of ten new cell lines from European ticks of the genera Argas, Dermacentor, Hyalomma, Ixodes and Rhipicephalus originating from Germany and Spain. For each cell line, the method used to generate the primary culture, a morphological description of the cells and species confirmation by sequencing of the partial 16S rRNA gene are presented. Further molecular analysis of the two new Ixodes ricinus cell lines and three existing cell lines of the same species revealed genetic variation between cell lines derived from ticks collected in the same or nearby locations. Collectively, these new cell lines will support research into a wide range of viral, bacterial and protozoal tick-borne diseases prevalent in Europe.

Item Type:	Article
Uncontrolled Keywords:	tick cell line, vector, Argas reflexus, Dermacentor reticulatus, Hyalomma lusitanicum, Hyalomma scupense, Ixodes ricinus, Rhipicephalus bursa
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	10 Jun 2022 13:42
Last Modified:	18 Jan 2023 21:00
DOI:	10.3390/microorganisms10061086
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3156183