Anderson, James R 
ORCID: 0000-0003-0489-7997, Jacobsen, Stine, Walters, Marie, Bundgaard, Louise, Diendorfer, Andreas, Hackl, Matthias, Clarke, Emily J, James, Victoria and Peffers, Mandy J ORCID: 0000-0001-6979-0440
(2022)
Small non-coding RNA landscape of extracellular vesicles from a post-traumatic model of equine osteoarthritis.
Frontiers in Veterinary Science, 9.
901269-.
Abstract
<jats:p>Extracellular vesicles comprise an as yet inadequately investigated intercellular communication pathway in the field of early osteoarthritis. We hypothesised that the small non-coding RNA expression pattern in synovial fluid and plasma would change during progression of experimental osteoarthritis. In this study, we conducted small RNA sequencing to provide a comprehensive overview of the temporal expression profiles of small non-coding transcripts carried by extracellular vesicles derived from plasma and synovial fluid for the first time in a posttraumatic model of equine osteoarthritis. Additionally, we characterised synovial fluid and plasma-derived extracellular vesicles with respect to quantity, size, and surface markers. The different temporal expressions of seven microRNAs in plasma and synovial fluid-derived extracellular vesicles, eca-miR-451, eca-miR-25, eca-miR-215, eca-miR-92a, eca-miR-let-7c, eca-miR-486-5p, and eca-miR-23a, and four snoRNAs, U3, snord15, snord46, and snord58, represent potential biomarkers for early osteoarthritis. Bioinformatics analysis of the differentially expressed microRNAs in synovial fluid highlighted that in early osteoarthritis these related to the inhibition of cell cycle, cell cycle progression, DNA damage and cell proliferation as well as increased cell viability and differentiation of stem cells. Plasma and synovial fluid-derived extracellular vesicle small non-coding signatures have been established for the first time in a temporal model of osteoarthritis. These could serve as novel biomarkers for evaluation of osteoarthritis progression or act as potential therapeutic targets.</jats:p>
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | osteoarthritis, extracellular vesicles, small non-coding RNA, synovial fluid, plasma |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 16 Aug 2022 10:14 |
| Last Modified: | 18 Jan 2023 20:47 |
| DOI: | 10.3389/fvets.2022.901269 |
| Open Access URL: | https://doi.org/10.3389/fvets.2022.901269 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3161375 |
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