Evidence of impaired mitochondrial cellular bioenergetics in ocular fibroblasts derived from glaucoma patients

Vallabh, Neeru A ORCID: 0000-0002-0109-4112, Armstrong, Jane, Czanner, Gabriela, McDonagh, Brian, Choudhary, Anshoo, Criddle, David N ORCID: 0000-0003-2952-8450 and Willoughby, Colin E (2022) Evidence of impaired mitochondrial cellular bioenergetics in ocular fibroblasts derived from glaucoma patients. FREE RADICAL BIOLOGY AND MEDICINE, 189. pp. 102-110.

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Official URL: http://dx.doi.org/10.1016/j.freeradbiomed.2022.07....

Abstract

Glaucoma is a progressive optic neuropathy characterized by the neurodegeneration of the retinal ganglion cells (RGCs) resulting in irreversible visual impairment and eventual blindness. RGCs are extremely susceptible to mitochondrial compromise due to their marked bioenergetic requirements and morphology. There is increasing interest in therapies targeting mitochondrial health as a method of preventing visual loss in managing glaucoma. The bioenergetic profile of Tenon's ocular fibroblasts from glaucoma patients and controls was investigated using the Seahorse XF24 analyser. Impaired mitochondrial cellular bioenergetics was detected in glaucomatous ocular fibroblasts including basal respiration, maximal respiration and spare capacity. Spare respiratory capacity levels reflect mitochondrial bio-energetic adaptability in response to pathophysiological stress. Basal oxidative stress was elevated in glaucomatous Tenon's ocular fibroblasts and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) induced reactive oxygen species (ROS) simulated the glaucomatous condition in normal Tenon's ocular fibroblasts. This work supports the role of therapeutic interventions to target oxidative stress or provide mitochondrial energetic support in glaucoma.

Item Type:	Article
Uncontrolled Keywords:	Tenon?s fibroblast, Glaucoma, Mitochondria, Seahorse XF analyser, Oxidative stress, Bioenergetics
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	18 Oct 2022 15:24
Last Modified:	18 Jan 2023 19:49
DOI:	10.1016/j.freeradbiomed.2022.07.009
Open Access URL:	https://doi.org/10.1016/j.freeradbiomed.2022.07.00...
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3165629