Rodriguez-Manzano, Jesus, Malpartida-Cardenas, Kenny, Moser, Nicolas, Pennisi, Ivana, Cavuto, Matthew, Miglietta, Luca, Moniri, Ahmad, Penn, Rebecca, Satta, Giovanni, Randell, Paul et al (show 5 more authors)
(2021)
Handheld Point-of-Care System for Rapid Detection of SARS-CoV-2 Extracted RNA in under 20 min.
ACS CENTRAL SCIENCE, 7 (2).
pp. 307-317.
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Abstract
The COVID-19 pandemic is a global health emergency characterized by the high rate of transmission and ongoing increase of cases globally. Rapid point-of-care (PoC) diagnostics to detect the causative virus, SARS-CoV-2, are urgently needed to identify and isolate patients, contain its spread and guide clinical management. In this work, we report the development of a rapid PoC diagnostic test (<20 min) based on reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) and semiconductor technology for the detection of SARS-CoV-2 from extracted RNA samples. The developed LAMP assay was tested on a real-time benchtop instrument (RT-qLAMP) showing a lower limit of detection of 10 RNA copies per reaction. It was validated against extracted RNA from 183 clinical samples including 127 positive samples (screened by the CDC RT-qPCR assay). Results showed 91% sensitivity and 100% specificity when compared to RT-qPCR and average positive detection times of 15.45 ± 4.43 min. For validating the incorporation of the RT-LAMP assay onto our PoC platform (RT-eLAMP), a subset of samples was tested (<i>n</i> = 52), showing average detection times of 12.68 ± 2.56 min for positive samples (<i>n</i> = 34), demonstrating a comparable performance to a benchtop commercial instrument. Paired with a smartphone for results visualization and geolocalization, this portable diagnostic platform with secure cloud connectivity will enable real-time case identification and epidemiological surveillance.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Prevention, Infectious Diseases, Emerging Infectious Diseases, Genetics, Clinical Research, Vaccine Related, Biodefense, Lung, 4 Detection, screening and diagnosis, 4.1 Discovery and preclinical testing of markers and technologies, Infection, 3 Good Health and Well Being |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 28 Oct 2022 14:30 |
| Last Modified: | 15 Mar 2024 14:11 |
| DOI: | 10.1021/acscentsci.0c01288 |
| Open Access URL: | https://pubs.acs.org/doi/pdf/10.1021/acscentsci.0c... |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3165831 |
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