Dewhurst, Robert E, Heinrich, Tatjana, Watt, Paul, Ostergaard, Paul, Marimon, Jose M, Moreira, Mariana, Houldsworth, Philip E, Rudrum, Jack D, Wood, David and Koks, Sulev 
ORCID: 0000-0001-6087-6643
(2022)
Validation of a rapid, saliva-based, and ultra-sensitive SARS-CoV-2 screening system for pandemic-scale infection surveillance.
SCIENTIFIC REPORTS, 12 (1).
5936-.
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Abstract
Without any realistic prospect of comprehensive global vaccine coverage and lasting immunity, control of pandemics such as COVID-19 will require implementation of large-scale, rapid identification and isolation of infectious individuals to limit further transmission. Here, we describe an automated, high-throughput integrated screening platform, incorporating saliva-based loop-mediated isothermal amplification (LAMP) technology, that is designed for population-scale sensitive detection of infectious carriers of SARS-CoV-2 RNA. Central to this surveillance system is the "Sentinel" testing instrument, which is capable of reporting results within 25 min of saliva sample collection with a throughput of up to 3840 results per hour. It incorporates continuous flow loading of samples at random intervals to cost-effectively adjust for fluctuations in testing demand. Independent validation of our saliva-based RT-LAMP technology on an automated LAMP instrument coined the "Sentinel", found 98.7% sensitivity, 97.6% specificity, and 98% accuracy against a RT-PCR comparator assay, confirming its suitability for surveillance screening. This Sentinel surveillance system offers a feasible and scalable approach to complement vaccination, to curb the spread of COVID-19 variants, and control future pandemics to save lives.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Saliva, Humans, RNA, Viral, Molecular Diagnostic Techniques, Sensitivity and Specificity, Nucleic Acid Amplification Techniques, Pandemics, COVID-19, SARS-CoV-2 |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 28 Oct 2022 15:25 |
| Last Modified: | 18 Jan 2023 19:48 |
| DOI: | 10.1038/s41598-022-08263-4 |
| Open Access URL: | https://www.nature.com/articles/s41598-022-08263-4 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3165868 |
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