Zhan, Dong, McConachie, Ellie, Edwards, Steven W 
ORCID: 0000-0002-7074-0552, Wright, Helen L ORCID: 0000-0003-0442-3134, Moots, Robert J ORCID: 0000-0001-7019-6211 and Honsawek, Sittisak
(2021)
Isolation of Microvesicles from Human Circulating Neutrophils.
BIO-PROTOCOL, 11 (19).
e3119-.
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Abstract
Neutrophil-derived microvesicles (NDMVs) are liberated by neutrophils upon cell activation by molecules. Once activated, neutrophils are primarily involved in acute inflammation; however, the microvesicles they produce are largely anti-inflammatory. NDMVs have been shown to protect cartilage during inflammatory arthritis. They exert these effects by inhibiting or affecting the function of target cells, including macrophages. NDMVs have the potential to act as disease-modifying agents, especially for inflammatory diseases. This protocol describes a method using differential centrifugation to separate neutrophils from whole human blood. Subsequently, neutrophils are identified by cytospin and Wright's staining, and then the NDMVs are isolated using differential centrifugation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Microvesicles, Neutrophils, Isolation, Differential centrifugation, Cytospin |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences Faculty of Health and Life Sciences > Clinical Directorate |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 01 Nov 2022 08:44 |
| Last Modified: | 18 Jan 2023 19:48 |
| DOI: | 10.21769/BioProtoc.3119 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3165912 |
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