"Fuel for the Damage Induced": Untargeted Metabolomics in Elite Rugby Union Match Play

Hudson, James F, Phelan, Marie M, Owens, Daniel J, Morton, James P, Close, Graeme L and Stewart, Claire E (2021) "Fuel for the Damage Induced": Untargeted Metabolomics in Elite Rugby Union Match Play. METABOLITES, 11 (8). 544-.

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Official URL: http://dx.doi.org/10.3390/metabo11080544

Abstract

The metabolic perturbations caused by competitive rugby are not well characterized. Our aim is to utilize untargeted metabolomics to develop appropriate interventions, based on the metabolic fluctuations that occur in response to this collision-based team sport. Seven members of an English Premiership rugby squad consented to provide blood, urine, and saliva samples daily, over a competitive week including gameday (GD), with physical demands and dietary intake also recorded. Sample collection, processing and statistical analysis were performed in accordance with best practice set out by the metabolomics standards initiative employing 700 MHz NMR spectroscopy. Univariate and multivariate statistical analysis were employed to reveal the acute energy needs of this high intensity sport are met via glycolysis, the TCA cycle and gluconeogenesis. The recovery period after cessation of match play and prior to training recommencing sees a re-entry to gluconeogenesis, coupled with markers of oxidative stress, structural protein degradation, and reduced fatty acid metabolism. This novel insight leads us to propose that effective recovery from muscle damaging collisions is dependent upon the availability of glucose. An adjustment in the periodisation of carbohydrate to increase GD+1 provision may prevent the oxidation of amino acids which may also be crucial to allay markers of structural tissue degradation. Should we expand the 'Fuel for the work required' paradigm in collision-based team sports to include 'Fuel for the damage induced'?

Item Type:	Article
Uncontrolled Keywords:	metabolomics, rugby, exercise, damage, collision
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	03 Nov 2022 14:56
Last Modified:	18 Jan 2023 19:48
DOI:	10.3390/metabo11080544
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3165981