Low-cost, high-throughput and rapid-prototyped 3D-integrated dielectrophoretic channels for continuous cell enrichment and separation

Faraghat, Shabnam AA, Fatoyinbo, Henry OO, Hoettges, Kai FF ORCID: 0000-0002-0415-1688 and Hughes, Michael Pycraft (2023) Low-cost, high-throughput and rapid-prototyped 3D-integrated dielectrophoretic channels for continuous cell enrichment and separation. ELECTROPHORESIS, 44 (11-12). pp. 947-955.

Text
Canyon paper final.docx - Author Accepted Manuscript
Download (1MB)

Official URL: http://dx.doi.org/10.1002/elps.202200234

Abstract

Microfluidic devices for dielectrophoretic cell separation are typically designed and constructed using microfabrication methods in a clean room, requiring time and expense. In this paper, we describe a novel alternative approach to microfluidic device manufacture, using chips cut from conductor-insulator laminates using a cutter plotter. This allows the manufacture of microchannel devices with micron-scale electrodes along every wall. Fabrication uses a conventional desktop cutter plotter, and requires no chemicals, masks or clean-room access; functional fluidic devices can be designed and constructed within a couple of hours at negligible cost. As an example, we demonstrate the construction of a continuous dielectrophoretic cell separator capable of enriching yeast cells to 80% purity at 10 000 cells/s.

Item Type:	Article
Uncontrolled Keywords:	continuous, dielectrophoresis, laminate, separation
Depositing User:	Symplectic Admin
Date Deposited:	08 Nov 2022 08:28
Last Modified:	11 Jul 2023 14:01
DOI:	10.1002/elps.202200234
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3166035