Zhao, Xichen, Zhang, Yuxin, Hang, Daiyun, Meng, Jia 
ORCID: 0000-0003-3455-205X and Wei, Zhen
(2022)
Detecting RNA modification using direct RNA sequencing: A systematic review.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, 20.
pp. 5740-5749.
|
PDF
Detecting RNA modification using direct RNA sequencing A systematic review.pdf - Published version Download (1MB) | Preview |
Abstract
Post-transcriptional RNA modifications are involved in a range of important cellular processes, including the regulation of gene expression and fine-tuning of the functions of RNA molecules. To decipher the context-specific functions of these post-transcriptional modifications, it is crucial to accurately determine their transcriptomic locations and modification levels under a given cellular condition. With the newly emerged sequencing technology, especially nanopore direct RNA sequencing, different RNA modifications can be detected simultaneously with a single molecular level resolution. Here we provide a systematic review of 15 published RNA modification prediction tools based on direct RNA sequencing data, including their computational models, input-output formats, supported modification types, and reported performances. Finally, we also discussed the potential challenges and future improvements of nanopore sequencing-based methods for RNA modification detection.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Biotechnology, Drug Abuse (NIDA only), Human Genome, Nanotechnology, Substance Misuse, Bioengineering, Genetics, 1.1 Normal biological development and functioning, 1 Underpinning research, Generic health relevance |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 21 Nov 2022 16:49 |
| Last Modified: | 15 Mar 2024 12:19 |
| DOI: | 10.1016/j.csbj.2022.10.023 |
| Open Access URL: | https://doi.org/10.1016/j.csbj.2022.10.023 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3166309 |
Dimensions
Dimensions
