Mwangi, Peter N, Page, Nicola A, Seheri, Mapaseka L, Mphahlele, M Jeffrey, Nadan, Sandrama, Esona, Mathew D, Kumwenda, Benjamin, Kamng'ona, Arox W 
ORCID: 0000-0002-0841-7586, Donato, Celeste M, Steele, Duncan A et al (show 4 more authors)
(2022)
Evolutionary changes between pre- and post- vaccine South African group A G2P[4] rotavirus strains, 2003-2017.
MICROBIAL GENOMICS, 8 (4).
000809-.
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Evolutionary changes between pre- and post-vaccine South African group A G2P[4] rotavirus strains, 2003-2017.pdf - Published version Download (1MB) | Preview |
Abstract
The transient upsurge of G2P[4] group A rotavirus (RVA) after Rotarix vaccine introduction in several countries has been a matter of concern. To gain insight into the diversity and evolution of G2P[4] strains in South Africa pre- and post-RVA vaccination introduction, whole-genome sequencing was performed for RVA positive faecal specimens collected between 2003 and 2017 and samples previously sequenced were obtained from GenBank (<i>n</i>=103; 56 pre- and 47 post-vaccine). Pre-vaccine G2 sequences predominantly clustered within sub-lineage IVa-1. In contrast, post-vaccine G2 sequences clustered mainly within sub-lineage IVa-3, whereby a radical amino acid (AA) substitution, S15F, was observed between the two sub-lineages. Pre-vaccine P[4] sequences predominantly segregated within sub-lineage IVa while post-vaccine sequences clustered mostly within sub-lineage IVb, with a radical AA substitution R162G. Both S15F and R162G occurred outside recognised antigenic sites. The AA residue at position 15 is found within the signal sequence domain of Viral Protein 7 (VP7) involved in translocation of VP7 into endoplasmic reticulum during infection process. The 162 AA residue lies within the hemagglutination domain of Viral Protein 4 (VP4) engaged in interaction with sialic acid-containing structure during attachment to the target cell. Free energy change analysis on VP7 indicated accumulation of stable point mutations in both antigenic and non-antigenic regions. The segregation of South African G2P[4] strains into pre- and post-vaccination sub-lineages is likely due to erstwhile hypothesized stepwise lineage/sub-lineage evolution of G2P[4] strains rather than RVA vaccine introduction. Our findings reinforce the need for continuous whole-genome RVA surveillance and investigation of contribution of AA substitutions in understanding the dynamic G2P[4] epidemiology.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | G2P[4] group A rotavirus strains, rotavirus, sub-lineages, whole-genome analysis |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 24 Nov 2022 10:18 |
| Last Modified: | 15 Mar 2024 04:24 |
| DOI: | 10.1099/mgen.0.000809 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3166356 |
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