Gallichan, Sarah, Perez-Sepulveda, Blanca M, Feasey, Nicholas A, Hinton, Jay CD 
ORCID: 0000-0003-2671-6026, Thomas, Juno and Smith, Anthony Marius
(2022)
Multiplex PCR Assay for Clade Typing of <i>Salmonella enterica</i> Serovar Enteritidis.
MICROBIOLOGY SPECTRUM, 10 (6).
e0318222-.
Abstract
Salmonella enterica serovar Enteritidis is one of the most commonly reported serovars of nontyphoidal Salmonella causing human disease and is responsible for both gastroenteritis and invasive nontyphoidal Salmonella (iNTS) disease worldwide. Whole-genome sequence (WGS) comparison of Salmonella Enteritidis isolates from across the world has identified three distinct clades, global epidemic, Central/East African, and West African, all of which have been implicated in epidemics: the global epidemic clade was linked to poultry-associated gastroenteritis, while the two African clades were related to iNTS disease. However, the distribution and epidemiology of these clades across Africa are poorly understood because identification of these clades currently requires whole-genome sequencing capacity. Here, we report a sensitive, time- and cost-effective real-time PCR assay capable of differentiating between the Salmonella Enteritidis clades to facilitate surveillance and to inform public health responses. The assay described here is limited to previously confirmed S. Enteritidis isolates. <b>IMPORTANCE</b> Challenges in the diagnosis and treatment of invasive Salmonella Enteritidis bloodstream infections in sub-Saharan Africa are responsible for a case fatality rate of approximately 15%. It is important to identify distinct clades of S. Enteritidis in diagnostic laboratories in the African setting to determine the different health outcomes associated with particular outbreaks. Here, we describe the development of a high-quality molecular classification assay for clade typing of S. Enteritidis that is ideal for use in public health laboratories in resource-limited settings.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | nontyphoidal Salmonella, real-time PCR, phylogeny, molecular surveillance |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 25 Nov 2022 10:29 |
| Last Modified: | 17 Oct 2023 11:32 |
| DOI: | 10.1128/spectrum.03182-22 |
| Open Access URL: | https://journals.asm.org/doi/10.1128/spectrum.0318... |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3166372 |
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