Obanda, Benear Apollo, Gibbons, Cheryl L, Fevre, Eric M 
ORCID: 0000-0001-8931-4986, Bebora, Lilly, Gitao, George, Ogara, William, Wang, Shu-Hua, Gebreyes, Wondwossen, Ngetich, Ronald, Blane, Beth et al (show 5 more authors)
(2022)
Multi-Drug Resistant <i>Staphylococcus aureus</i> Carriage in Abattoir Workers in Busia, Kenya.
ANTIBIOTICS-BASEL, 11 (12).
1726-.
Abstract
Abattoir workers have been identified as high-risk for livestock-associated Staphylococcus aureus carriage. This study investigated S. aureus carriage in abattoir workers in Western Kenya. Nasal swabs were collected once from participants between February-November 2012. S. aureus was isolated using bacterial culture and antibiotic susceptibility testing performed using the VITEK 2 instrument and disc diffusion methods. Isolates underwent whole genome sequencing and Multi Locus Sequence Types were derived from these data. S. aureus (n = 126) was isolated from 118/737 (16.0%) participants. Carriage was higher in HIV-positive (24/89, 27.0%) than HIV−negative participants (94/648, 14.5%; p = 0.003). There were 23 sequence types (STs) identified, and half of the isolates were ST152 (34.1%) or ST8 (15.1%). Many isolates carried the Panton-Valentine leucocidin toxin gene (42.9%). Only three isolates were methicillin resistant S. aureus (MRSA) (3/126, 2.4%) and the prevalence of MRSA carriage was 0.4% (3/737). All MRSA were ST88. Isolates from HIV-positive participants (37.0%) were more frequently resistant to sulfamethoxazole/trimethoprim compared to isolates from HIV-negative participants (6.1%; p < 0.001). Similarly, trimethoprim resistance genes were more frequently detected in isolates from HIV-positive (81.5%) compared to HIV-negative participants (60.6%; p = 0.044). S. aureus in abattoir workers were representative of major sequence types in Africa, with a high proportion being toxigenic isolates. HIV-positive individuals were more frequently colonized by antimicrobial resistant S. aureus which may be explained by prophylactic antimicrobial use.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | S, aureus, MSSA, MRSA, abattoir, slaughterhouse, Kenya, HIV, AMR, antimicrobial resistance |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 01 Dec 2022 09:12 |
| Last Modified: | 18 Oct 2023 14:31 |
| DOI: | 10.3390/antibiotics11121726 |
| Open Access URL: | https://doi.org/10.3390/antibiotics11121726 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3166459 |
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