New Cell Lines Derived from Laboratory Colony Triatoma infestans and Rhodnius prolixus, Vectors of Trypanosoma cruzi, Do Not Harbour Triatoma Virus



Penrice-Randal, Rebekah ORCID: 0000-0002-0653-2097, Hartley, Catherine, Beliavskaia, Alexandra, Dong, Xiaofeng, Brandner-Garrod, Luke, Whitten, Miranda and Bell-Sakyi, Lesley ORCID: 0000-0002-7305-0477
(2022) New Cell Lines Derived from Laboratory Colony Triatoma infestans and Rhodnius prolixus, Vectors of Trypanosoma cruzi, Do Not Harbour Triatoma Virus. INSECTS, 13 (10). 906-.

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Abstract

Triatomine bugs of the genera <i>Triatoma</i> and <i>Rhodnius</i> are vectors of Chagas disease, a neglected tropical disease of humans in South America caused by <i>Trypanosoma cruzi</i>. Triatoma virus (TrV), a natural pathogen of <i>Triatoma infestans</i>, has been proposed as a possible tool for the bio-control of triatomine bugs, but research into this virus has been hampered by a lack of suitable host cells for <i>in vitro</i> propagation. Here we report establishment and partial characterisation of continuous cell lines from embryos of <i>T. infestans</i> (TIE/LULS54) and <i>Rhodnius prolixus</i> (RPE/LULS53 and RPE/LULS57). RNAseq screening by a sequence-independent, single primer amplification approach confirmed the absence of TrV and other RNA viruses known to infect <i>R. prolixus</i>, indicating that these new cell lines could be used for propagation of TrV.

Item Type: Article
Uncontrolled Keywords: cell line, insect, kissing bug, Rhodnius prolixus, Triatoma infestans, Triatoma virus, vector
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User: Symplectic Admin
Date Deposited: 20 Dec 2022 08:38
Last Modified: 20 Dec 2022 08:39
DOI: 10.3390/insects13100906
Open Access URL: http://10.0.13.62/insects13100906
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3166723