Penrice-Randal, Rebekah ORCID: 0000-0002-0653-2097, Hartley, Catherine, Beliavskaia, Alexandra, Dong, Xiaofeng, Brandner-Garrod, Luke, Whitten, Miranda and Bell-Sakyi, Lesley ORCID: 0000-0002-7305-0477
(2022)
New Cell Lines Derived from Laboratory Colony Triatoma infestans and Rhodnius prolixus, Vectors of Trypanosoma cruzi, Do Not Harbour Triatoma Virus.
INSECTS, 13 (10).
906-.
Abstract
Triatomine bugs of the genera <i>Triatoma</i> and <i>Rhodnius</i> are vectors of Chagas disease, a neglected tropical disease of humans in South America caused by <i>Trypanosoma cruzi</i>. Triatoma virus (TrV), a natural pathogen of <i>Triatoma infestans</i>, has been proposed as a possible tool for the bio-control of triatomine bugs, but research into this virus has been hampered by a lack of suitable host cells for <i>in vitro</i> propagation. Here we report establishment and partial characterisation of continuous cell lines from embryos of <i>T. infestans</i> (TIE/LULS54) and <i>Rhodnius prolixus</i> (RPE/LULS53 and RPE/LULS57). RNAseq screening by a sequence-independent, single primer amplification approach confirmed the absence of TrV and other RNA viruses known to infect <i>R. prolixus</i>, indicating that these new cell lines could be used for propagation of TrV.
Item Type: | Article |
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Uncontrolled Keywords: | cell line, insect, kissing bug, Rhodnius prolixus, Triatoma infestans, Triatoma virus, vector |
Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
Depositing User: | Symplectic Admin |
Date Deposited: | 20 Dec 2022 08:38 |
Last Modified: | 20 Dec 2022 08:39 |
DOI: | 10.3390/insects13100906 |
Open Access URL: | http://10.0.13.62/insects13100906 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3166723 |