Development and Characterization of a Luminescence-Based High-Throughput Serum Bactericidal Assay (L-SBA) to Assess Bactericidal Activity of Human Sera against Nontyphoidal <i>Salmonella</i>

Collapse authors list.
Aruta, Maria Grazia, De Simone, Daniele, Dale, Helen, Chirwa, Esmelda, Kadwala, Innocent, Mbewe, Maurice, Banda, Happy, Gordon, Melita ORCID: 0000-0002-0629-0884, Pizza, Mariagrazia, Berlanda Scorza, Francesco et al (show 3 more authors) , Nyirenda, Tonney, Canals, Rocio and Rossi, Omar (2022) Development and Characterization of a Luminescence-Based High-Throughput Serum Bactericidal Assay (L-SBA) to Assess Bactericidal Activity of Human Sera against Nontyphoidal <i>Salmonella</i>. METHODS AND PROTOCOLS, 5 (6). 100-.

PDF Development and Characterization of a Luminescence-Based High-Throughput Serum Bactericidal Assay (L-SBA) to Assess Bacteric.pdf - Published version Download (2MB) | Preview

Abstract

<i>Salmonella</i> Typhimurium and <i>Salmonella</i> Enteritidis are leading causative agents of invasive nontyphoidal <i>Salmonella</i> (iNTS) disease, which represents one of the major causes of death and morbidity in sub-Saharan Africa, still partially underestimated. Large sero-epidemiological studies are necessary to unravel the burden of disease and guide the introduction of vaccines that are not yet available. Even if no correlate of protection has been determined so far for iNTS, the evaluation of complement-mediated functionality of antibodies generated towards natural infection or elicited upon vaccination may represent a big step towards this achievement. Here we present the setup and the intra-laboratory characterization in terms of repeatability, intermediate precision, linearity, and specificity of a high-throughput luminescence-based serum bactericidal assay (L-SBA). This method could be useful to perform sero-epidemiological studies across iNTS endemic countries and for evaluation of antibodies raised against iNTS vaccine candidates in upcoming clinical trials.

Item Type:	Article
Uncontrolled Keywords:	generalized modules for membrane antigens (GMMA), serum bactericidal assay (SBA), nontyphoidal Salmonella, functional assay, human sera, antibodies, vaccine, iNTS
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	20 Jan 2023 14:29
Last Modified:	18 Oct 2023 17:19
DOI:	10.3390/mps5060100
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3167767